CD23 / FCER2 recombinant protein

Recombinant Mouse CD23 / FCER2 Protein (His tag)

Cat. Num. AAA2546842

• Gene Names: Fcer2a; CD23; Fce2; Fcer2; Ly-42
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: CD23 / FCER2; Recombinant Mouse CD23 / FCER2 Protein (His tag); Fcer2a; CD23; Fce2; Fcer2; Ly-42; CD23 / FCER2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 330

• Application Notes: The recombinant mouse FCER2A comprises 301 amino acids and predicts a molecular mass of 34.3 kDa. As a result of glycosylation, the apparent molecular mass of mouse FCER2A is approximately 40-45 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD23 / FCER2 recombinant protein

Background: Fc fragment of IgE, low affinity II, receptor for (CD23) or CD23 antigen is a member of the cluster of differentiation family. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Physiologically, CD molecules can act in numerous ways, often acting as receptors or ligands (the molecule that activates a receptor) important to the cell. A signal cascade is usually initiated, altering the behavior of the cell (see cell signaling). Some CD proteins do not play a role in cell signaling, but have other functions, such as cell adhesion. CD23/FCER2 is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Increased levels of soluble CD23/FCER2 cause the recruitment of non-sensitised B-cells in the presentation of antigen peptides to allergen-specific B-cells, therefore increasing the production of allergen specific IgE. IgE, in turn, is known to upregulate the cellular expression of CD23 and Fc epsilon RI (high-affinity IgE receptor).

Description: A DNA sequence encoding the extracellular domain of mouse FCER2A isoform A (P20693-1) (Glu 51-Pro 331) was fused with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 359339166
• NCBI GeneID: 14128
• NCBI Accession #: NP_001240666.1
• NCBI GenBank Nucleotide #: NM_001253737.1
• UniProt Accession #: P20693; Q61556; Q61557
• Molecular Weight: 37,487 Da
• NCBI Official Full Name: low affinity immunoglobulin epsilon Fc receptor isoform B
• NCBI Official Synonym Full Names: Fc receptor, IgE, low affinity II, alpha polypeptide
• NCBI Official Symbol: Fcer2a
• NCBI Official Synonym Symbols: CD23; Fce2; Fcer2; Ly-42
• NCBI Protein Information: low affinity immunoglobulin epsilon Fc receptor
• UniProt Protein Name: Low affinity immunoglobulin epsilon Fc receptor
• UniProt Gene Name: Fcer2
• UniProt Synonym Gene Names: Fcer2a

Uniprot Description

FCER2: Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8 A1.1|8 1.92 cM

Cellular Component: external side of plasma membrane

Biological Process: positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of killing of cells of another organism; positive regulation of nitric-oxide synthase activity; positive regulation of nitric-oxide synthase biosynthetic process