VDR / NR1I1 recombinant protein

Recombinant Mouse VDR / NR1I1 Protein (His tag)

Cat. Num. AAA2546657

• Gene Names: Vdr; Nr1i1
• Purity: > 86 % as determined by SDS-PAGE
• Synonyms: VDR / NR1I1; Recombinant Mouse VDR / NR1I1 Protein (His tag); VDR; Nr1i1; VDR / NR1I1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 86 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
• Sequence Length: 422

• Application Notes: The recombinant mouse VDR consists of 432 amino acids and has a calculated molecular mass of 49.2 kDa. The recombinant protein migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for VDR / NR1I1 recombinant protein

Background: VDR (vitamin D (1, 25- dihydroxyvitamin D3)receptor), also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Vitamin D receptors (VDRs) are members of the NR1I family, which also includes pregnane X (PXR) and constitutive androstane (CAR) receptors, that form heterodimers with members of the retinoid X receptor family. VDRs repress expression of 1alpha-hydroxylase (the proximal activator of 1, 25 (OH)2D3) and induce expression of the 1, 25 (OH)2D3 inactivating enzyme CYP24. Also, it has recently been identified as an additional bile acid receptor alongside FXR and may function to protect gut against the toxic and carcinogenic effects of these endobiotics. VDR is expressed in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. It is the nuclear hormone receptor, also called transcription factor that mediates the action of vitamin D3. Inherited mutations in the VDR gene leads to rickets.

Description: A DNA sequence encoding the mouse VDR (P48281) (Met1-Ser422) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 31543944
• NCBI GeneID: 22337
• NCBI Accession #: NP_033530.2
• NCBI GenBank Nucleotide #: NM_009504.4
• UniProt Accession #: P48281; Q922X0
• Molecular Weight: 47,834 Da
• NCBI Official Full Name: vitamin D3 receptor
• NCBI Official Synonym Full Names: vitamin D receptor
• NCBI Official Symbol: Vdr
• NCBI Official Synonym Symbols: Nr1i1
• NCBI Protein Information: vitamin D3 receptor
• UniProt Protein Name: Vitamin D3 receptor
• UniProt Gene Name: Vdr
• UniProt Synonym Gene Names: Nr1i1; VDR

Uniprot Description

VDR: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. Defects in VDR are the cause of rickets vitamin D- dependent type 2A (VDDR2A). A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: DNA-binding; Nuclear receptor; Transcription factor

Chromosomal Location of Human Ortholog: 15|15 F1

Cellular Component: caveola; cytosol; dense fibrillar component; euchromatin; heterochromatin; intracellular membrane-bound organelle; nuclear heterochromatin; nuclear matrix; nucleoplasm; nucleus; perinuclear region of cytoplasm; receptor complex; T-tubule

Molecular Function: DNA binding; ligand-dependent nuclear receptor activity; protein binding; retinoid X receptor binding; sequence-specific DNA binding; transcription factor activity; vitamin D binding; vitamin D3 receptor activity

Biological Process: calcium ion transport; cell morphogenesis; cellular calcium ion homeostasis; intestinal absorption; lactation; multicellular organismal development; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; organ morphogenesis; positive regulation of apoptosis involved in mammary gland involution; positive regulation of keratinocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of calcium ion transport; regulation of transcription, DNA-dependent; skeletal development