Cathepsin V / Cathepsin L2 / Preproprotein Recombinant Protein | CTSV recombinant protein

Recombinant Human Cathepsin V / Cathepsin L2 / Preproprotein Protein (His tag)

Cat. Num. AAA2546534

• Gene Names: CTSV; CTSU; CATL2; CTSL2
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Cathepsin V / Cathepsin L2 / Preproprotein; Recombinant Human Cathepsin V / Cathepsin L2 / Preproprotein Protein (His tag); CTSU; CTSV; CATL2; MGC125957; CTSL2; CTSV recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 334

• Application Notes: The recombinant pro form of human cathepsin V comprises 328 amino acids and predicts a molecular mass of 37.1 kDa. As a result of glycosylation, it migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Val 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CTSV recombinant protein

Background: Cathepsin V (CTSV), also known as Cathepsin L2, CTSL2, and CATL2, is a member of the peptidase C1 family. It is predominantly expressed in the thymus and testis. Cathepsin V is also expressed in corneal epithelium, and to a lesser extent in conjuctival epithelium and skin. It is a lysosomal cysteine proteinase that may play an important role in corneal physiology. It has about 75% protein sequence identity to murine cathepsin L. The fold of this enzyme is similar to the fold adopted by other members of the papain superfamily of cysteine proteases. Cathepsin V has been recently described as highly homologous to Cathepsin L and exclusively expressed in human thymus and testis. Cathepsin V is the dominant cysteine protease in cortical human thymic epithelial cells, while Cathepsin L and Cathepsin S seem to be restricted to dendritic and macrophage-like cells. Active Cathepsin V in thymic lysosomal preparations was demonstrated by active-site labeling. Recombinant Cathepsin V was capable of converting Ii into CLIP efficiently, suggesting that it is the protease that controls the generation of alphabeta-CLIP complexes in the human thymus. Cathepsin V is the third elastolytic cysteine protease which exhibits the most potent elastase activity yet described among human proteases and that it is present in atherosclerotic plaque specimens. Cathepsin L2 may play a specialized role in the thymus and testis. Expression analysis of cathepsin L2 in human tumors revealed a widespread expression in colorectal and breast carcinomas but not in normal colon or mammary gland or in peritumoral tissues. Cathepsin L2 was also expressed by colorectal and breast cancer cell lines as well as by some tumors of diverse origin, including ovarian and renal carcinomas.

Description: A DNA sequence encoding the full length of human cathepsin L2 (NP_001324.2) (Met 1-Val 334) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 23110960
• NCBI GeneID: 1515
• NCBI Accession #: NP_001324.2
• NCBI GenBank Nucleotide #: NM_001333.3
• UniProt Accession #: O60911; O60233; Q2TB86; Q5T1U0
• Molecular Weight: 37,329 Da
• NCBI Official Full Name: cathepsin L2 preproprotein
• NCBI Official Synonym Full Names: cathepsin V
• NCBI Official Symbol: CTSV
• NCBI Official Synonym Symbols: CTSU; CATL2; CTSL2
• NCBI Protein Information: cathepsin L2
• UniProt Protein Name: Cathepsin L2
• UniProt Gene Name: CTSV
• UniProt Synonym Gene Names: CATL2; CTSL2; CTSU

NCBI Description

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2011]

Uniprot Description

CTSL2: Cysteine protease. May have an important role in corneal physiology. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.15; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q22.33

Cellular Component: extracellular region; extracellular space; lysosomal lumen; lysosome

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; extracellular matrix disassembly; proteolysis involved in cellular protein catabolic process