SDS-PAGE
JAML / AMICA recombinant protein
Recombinant Human JAML / AMICA Protein (His tag)
Gene Names
JAML; AMICA; Gm638; AMICA1; CREA7-1; CREA7-4
Purity
> 90 % as determined by SDS-PAGE
Synonyms
JAML / AMICA; Recombinant Human JAML / AMICA Protein (His tag); JAML; AMICA; Gm638; CREA7-1; CREA7-4; FLJ37080; MGC118814; MGC118815; AMICA1; JAML / AMICA recombinant protein
Host
Human Cells
Purity/Purification
> 90 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
394
Application Notes
The secreted recombinant human JAML consists of 267 amino acids with the predicted molecular mass of 30.5 kDa. As a result of glycosylation, rhJAML migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Leu 20
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for JAML / AMICA recombinant protein
Background: Junctional adhesion molecules (JAMs) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites. JAML (Junctional adhesion molecule-like), also known as AMICA1 (Adhesion molecule interacting with CXADR antigen 1), a protein related to the JAM family, is restricted to leukocytes and promotes their adhesion to endothelial cells. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail involved in activation signaling. Monocytic JAML/AMICA1 plays a critical role in regulating monocyte transendothelial migration (TEM) probably via binding to the endothelial coxsackie and adenovirus receptor (CAR) and other tight junction-associated adhesive molecules. The Expression of JAML/AMICA1 is restricted to the hematopoietic tissues with the exception of liver. JAML may function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, JAML/AMICA1 also enhances myeloid leukemia cell adhesion to endothelial cells.
Description: A DNA sequence encoding the human JAML (NP_001091996.1) extracellular domain (Met 1-Leu 275) was fused with a polyhistidine tag at the C-terminus.
Description: A DNA sequence encoding the human JAML (NP_001091996.1) extracellular domain (Met 1-Leu 275) was fused with a polyhistidine tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,136 Da
NCBI Official Full Name
junctional adhesion molecule-like isoform 1
NCBI Official Synonym Full Names
junction adhesion molecule like
NCBI Official Symbol
JAML
NCBI Official Synonym Symbols
AMICA; Gm638; AMICA1; CREA7-1; CREA7-4
NCBI Protein Information
junctional adhesion molecule-like
UniProt Protein Name
Junctional adhesion molecule-like
UniProt Gene Name
JAML
Uniprot Description
AMICA1: an adhesion molecule that may function in the migration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells. Up-regulated upon retinoic acid, Me2SO and PMA treatment in differentiating myeloid leukemia cells. Three alternatively spliced isoforms have been described.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: plasma membrane; tight junction
Molecular Function: cell adhesion molecule binding; integrin binding; protein homodimerization activity
Biological Process: gamma-delta T cell activation; heterophilic cell adhesion; leukocyte migration; neutrophil chemotaxis; positive regulation of epithelial cell proliferation involved in wound healing; regulation of immune response
Research Articles on JAML / AMICA
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Product Notes
The JAML / AMICA jaml (Catalog #AAA2546511) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant human JAML consists of 267 amino acids with the predicted molecular mass of 30.5 kDa. As a result of glycosylation, rhJAML migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the JAML / AMICA jaml for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAML / AMICA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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