SLITRK1 recombinant protein

Recombinant Human SLITRK1 Protein (His & Fc tag)

Cat. Num. AAA2546320

• Gene Names: SLITRK1; TTM; LRRC12
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: SLITRK1; Recombinant Human SLITRK1 Protein (His & Fc tag); FLJ54428; KIAA0918; KIAA1910; LRRC12; RP11-395N17.1; SLITRK1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
• Sequence Length: 696

• Application Notes: The recombinant human SLITRK1/Fc chimera is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 846 amino acids and predicts a molecular mass of 95 kDa. As a result of glycosylation, the apparent molecular mass of rhSLITRK1/Fc monomer is approximately 130-150 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Asn 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SLITRK1 recombinant protein

Background: SLITRK1 (Slit and Trk-like family member 1) is a integral membrane protein belonging to the SLITRK family consists of at least 6 members (SLITRK1-6). They are named and characterized by the presence of two leucine-rich repeats (LRRs) in the extracellular domain similar to those found in a secreted axonal growth-controlling protein, Slit, as well as a C-terminal domain with homology to Trk neurotrophin tyrosine kinase receptors. Expression of SLITRKs are highly restricted to neural tissues, and are identified as the neuronal components modulating the neurite outgrowth. More specifically, SLITRK1 expression is found in the mature neurons of the cerebrum, thalamus and hippocampus, and induces unipolar neurites in cultured neuronal cells. Human SLITRK1 is a 696 amino acid precursor protein, and one truncating frameshift mutation (448 aa) has been linked to Tourette's syndrome, a genetically influenced developmental neuropsychiatric disorder characterized by chronic vocal and motor tics. In addition, all SLITRK genes are differentially expressed in brain tumors, such as astrocytoma, oligodendroglioma, glioblastoma, and are suggested to be useful molecular indicators of brain tumor properties.

Description: A DNA sequence encoding the extracellular domain (Met 1-Ser 616) of human SLITRK1 (NP_443142.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 40217817
• NCBI GeneID: 114798
• NCBI Accession #: NP_443142.1
• NCBI GenBank Nucleotide #: NM_052910.2
• UniProt Accession #: Q96PX8; Q5U5I6; Q96SF9
• Molecular Weight: 77,735 Da
• NCBI Official Full Name: SLIT and NTRK-like protein 1
• NCBI Official Synonym Full Names: SLIT and NTRK like family member 1
• NCBI Official Symbol: SLITRK1
• NCBI Official Synonym Symbols: TTM; LRRC12
• NCBI Protein Information: SLIT and NTRK-like protein 1
• UniProt Protein Name: SLIT and NTRK-like protein 1
• Protein Family: SLIT and NTRK-like protein
• UniProt Gene Name: SLITRK1
• UniProt Synonym Gene Names: KIAA1910; LRRC12

NCBI Description

This gene encodes a member of the SLITRK protein family. Members of this family are integral membrane proteins that are characterized by two N-terminal leucine-rich repeat (LRR) domains and a C-terminal region that shares homology with trk neurotrophin receptors. However, the protein encoded by this gene lacks the region of homology to neurotrophin receptors. This protein is thought to be involved in neurite outgrowth. Mutations in this gene may be associated with Tourette syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

SLITRK1: Enhances neuronal dendrite outgrowth. Defects in SLITRK1 may be a cause of Gilles de la Tourette syndrome (GTS). GTS is a neurologic disorder manifested particularly by motor and vocal tics and associated with behavioral abnormalities. Defects in SLITRK1 may be a cause of trichotillomania (TTM). It is a neuropsychiatric disorder characterized by chronic, repetitive, or compulsive hair pulling resulting in noticeable hair loss. Affected individuals may develop physical complications and often have overlapping psychologic disorders, such as Gilles de la Tourette syndrome or obsessive-compulsive disorder. Belongs to the SLITRK family.

Protein type: Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q31.1

Biological Process: axonogenesis

Disease: Gilles De La Tourette Syndrome; Trichotillomania