ECE1 recombinant protein

Recombinant Human ECE1 Protein (His tag)

Cat. Num. AAA2546028

• Gene Names: ECE1; ECE
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: ECE1; Recombinant Human ECE1 Protein (His tag); RP3-329E20.1; ECE; ECE1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 758

• Application Notes: The recombinant human ECE1 comprises 698 amino acids and has a predicted molecular mass of 80 kDa. As a result of glycosylation, rh ECE1 migrates as an approximately 125 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ECE1 recombinant protein

Background: Endothelin-converting enzyme 1, also known as ECE-1, is a single-pass type I I membrane protein which belongs to the peptidase M13 family. ECE-1 converts big endothelin-1 to endothelin-1. ECE-1 is a membrane metalloprotease that generates endothelin from its direct precursor big endothelin. Four isoforms of ECE-1 are produced from a single gene through the use of alternate promoters. These isoforms share the same extracellular catalytic domain and contain unique cytosolic tails, which results in their specific subcellular targeting.All isoforms of ECE-1 are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C of ECE-1 are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C of ECE-1 in liver, testis and small intestine; isoform B, C and D of ECE-1 in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Defects in ECE1 are a cause of Hirschsprung disease, cardiac defects and autonomic dysfunction. It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction.

Description: A DNA sequence encoding the extracellular domain of human ECE1 isoform B (P42892-1) (Gln 90-Trp 770) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 164519136
• NCBI GeneID: 1889
• NCBI Accession #: NP_001106818.1
• NCBI GenBank Nucleotide #: NM_001113347.1
• UniProt Accession #: P42892; Q14217; Q17RN5; Q2Z2K8; Q58GE7; Q5THM5; Q5THM7; Q5THM8; Q9UJQ6; Q9UPF4; A8K3P1; B4E291
• Molecular Weight: 87,022 Da
• NCBI Official Full Name: endothelin-converting enzyme 1 isoform 3
• NCBI Official Synonym Full Names: endothelin converting enzyme 1
• NCBI Official Symbol: ECE1
• NCBI Official Synonym Symbols: ECE
• NCBI Protein Information: endothelin-converting enzyme 1
• UniProt Protein Name: Endothelin-converting enzyme 1
• Protein Family: Endothelin-converting enzyme
• UniProt Gene Name: ECE1
• UniProt Synonym Gene Names: ECE-1

NCBI Description

The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

ECE1: Converts big endothelin-1 to endothelin-1. Defects in ECE1 are a cause of Hirschsprung disease cardiac defects and autonomic dysfunction (HSCRCDAD). It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction. Belongs to the peptidase M13 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; EC 3.4.24.71; Membrane protein, integral; Protease; Vesicle

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: endosome; external side of plasma membrane; integral to membrane; lysosomal membrane; membrane; perinuclear region of cytoplasm; vesicle

Molecular Function: endopeptidase activity; metalloendopeptidase activity; peptide hormone binding; protein binding; protein homodimerization activity

Biological Process: ear development; embryonic digit morphogenesis; heart development; hormone catabolic process; peptide hormone processing; positive regulation of receptor recycling; protein processing; regulation of systemic arterial blood pressure by endothelin; regulation of vasoconstriction

Disease: Hirschsprung Disease, Cardiac Defects, And Autonomic Dysfunction; Hypertension, Essential