AGO2 / Argonaute 2 / EIF2C2 Active Protein | AGO2 active protein

Recombinant Human AGO2/Argonaute 2/EIF2C2 Protein (His Tag)(Active)

Cat. Num. AAA2545928

• Gene Names: AGO2; PPD; Q10; CASC7; EIF2C2; LINC00980
• Purity: >85 % as determined by reducing SDS-PAGE.
• Synonyms: AGO2 / Argonaute 2 / EIF2C2; Recombinant Human AGO2/Argonaute 2/EIF2C2 Protein (His Tag)(Active); Argonaute 2; EIF2C2; Q10; AGO2 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.5, 10% glycerol, 0.5mM TCEP, 5% Trehalose, 5% Mannitol, 0.01%Tween 80.
• Sequence Positions: Met 1-Ala 859

• Species: Human
• Tag: N-His
• Bio_activity: Human AGO2 can bind Let-7a RNA and cleave target RNA (21nt).
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: It is recommended that sterile water (70uL) be added to the vial to prepare a stock solution of 0.15mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for AGO2 active protein

Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 29171734
• NCBI GeneID: 27161
• NCBI Accession #: NP_036286.2
• NCBI GenBank Nucleotide #: NM_012154.4
• Molecular Weight: Mol_Mass: 99 kDa; AP_Mol_Mass: 99 kDa
• NCBI Official Full Name: protein argonaute-2 isoform 1
• NCBI Official Synonym Full Names: argonaute 2, RISC catalytic component
• NCBI Official Symbol: AGO2
• NCBI Official Synonym Symbols: PPD; Q10; CASC7; EIF2C2; LINC00980
• NCBI Protein Information: protein argonaute-2
• UniProt Protein Name: Protein argonaute-2
• Protein Family: Protein argonaute
• UniProt Gene Name: AGO2
• UniProt Synonym Gene Names: EIF2C2; Argonaute2; hAgo2; eIF-2C 2; eIF2C 2; PPD

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: EC 3.1.26.n2; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm; cytosol; membrane; mRNA cap complex; nucleoplasm; nucleus; polysome; ribonucleoprotein complex

Molecular Function: double-stranded RNA binding; endoribonuclease activity; miRNA binding; protein binding; protein C-terminus binding; RNA 7-methylguanosine cap binding; single-stranded RNA binding; siRNA binding

Biological Process: miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; negative regulation of translational initiation; phosphoinositide-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; pre-microRNA processing; RNA interference, siRNA loading onto RISC; RNA secondary structure unwinding; RNA-mediated gene silencing; RNA-mediated posttranscriptional gene silencing; Wnt receptor signaling pathway, calcium modulating pathway