DOT1L / KMT4 recombinant protein

Recombinant Human DOT1L / KMT4 Protein

Cat. Num. AAA2545753

• Gene Names: DOT1L; DOT1; KMT4
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: DOT1L / KMT4; Recombinant Human DOT1L / KMT4 Protein; DKFZp586P1823; DOT1; KIAA1814; KMT4; DOT1L / KMT4 recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM HEPES, 150mM NaCl, 1mM EDTA, 15% glycerol, pH 7.5
• Sequence Length: 1537

• Application Notes: The recombinant human DOT1L comprises 417 amino acids and has a predicted molecular mass of 47.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions as predicted.
• Predicted N Terminal: Gly
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for DOT1L / KMT4 recombinant protein

Background: Histone-lysine N-methyltransferase, H3 lysine-79 specific, also known as Histone H3-K79 methyltransferase, DOT1-like protein, Lysine N-methyltransferase 4 and DOT1L, is a nucleus protein which belongs to the DOT1 family. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones. DOT1L is an histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA. Methylation of lysine 79 on histone H3 (H3K79) is mediated by DOT1L. It is involved in the regulation of telomeric silencing, development, cell cycle checkpoint and transcription. Mass spectrometry of the DOT1L-containing complex revealed that AF9, ENL and NPM1 were shown to be major DOT1L-interacting proteins. DOT1L might control AF9- and ENL-mediated transcription, regulate RNA processing, and function as a histone chaperone in a NPM1-dependent manner.

Description: A DNA sequence encoding the human DOT1L (NP_115871.1) N-terminal segment (Gly 2-Lys 416) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 22094135
• NCBI GeneID: 84444
• NCBI Accession #: NP_115871.1
• NCBI GenBank Nucleotide #: NM_032482.2
• UniProt Accession #: Q8TEK3; O60379; Q96JL1
• Molecular Weight: 164,856 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase, H3 lysine-79 specific
• NCBI Official Synonym Full Names: DOT1 like histone lysine methyltransferase
• NCBI Official Symbol: DOT1L
• NCBI Official Synonym Symbols: DOT1; KMT4
• NCBI Protein Information: histone-lysine N-methyltransferase, H3 lysine-79 specific
• UniProt Protein Name: Histone-lysine N-methyltransferase, H3 lysine-79 specific
• UniProt Gene Name: DOT1L
• UniProt Synonym Gene Names: KIAA1814; KMT4; H3-K79-HMTase

NCBI Description

The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]

Uniprot Description

DOT1L: a protein-lysine N-methyltransferase of the seven beta-strand class. Catalyzes the mono-, di- and tri-methylation of H3K79. Methylated H3K79 is associated with active gene transcription. DOT1L activity drives proliferation of mixed lineage leukemia 1 protein (MLL)-rearranged leukemias, inducing the aberrant transcription of a number of leukemogenic genes including HOXA9 and MEIS1. Binds to DNA. Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; nucleus; protein complex

Molecular Function: histone lysine N-methyltransferase activity (H3-K79 specific); histone methyltransferase activity; histone-lysine N-methyltransferase activity; protein binding

Biological Process: chromatin silencing at telomere; DNA damage checkpoint; DNA repair; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of JAK-STAT cascade; telomere organization and biogenesis