TIM4 / TIMD4 recombinant protein

Recombinant Human TIM4 / TIMD4 Protein (His tag)

Cat. Num. AAA2545557

• Gene Names: TIMD4; TIM4; SMUCKLER
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Synonyms: TIM4 / TIMD4; Recombinant Human TIM4 / TIMD4 Protein (His tag); FLJ27515; SMUCKLER; TIM4; TIM4 / TIMD4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 90 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Met 1-Leu 315

• Application Notes: The recombinant human TIMD4 consists of 302 amino acids and has a predicted molecular mass of 32.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTIMD4 is approximately 60-65 kDa due to glycosylation.
• Predicted N Terminal: Glu 25
• Species: Human
• Reconstitution: It is recommended that sterile water(200 ?l) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Endotoxin: < 1.0 EU per ?g as determined by the LAL method.
• Tag: C-His
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C.; Store it under sterile conditions at -20 degree C to -80 degree C.; It is recommended that the protein be aliquoted for optimal storage.; Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for TIM4 / TIMD4 recombinant protein

A type I transmembrane protein called TIM4 (T-cell immunoglobulin- and mucin-domain-containing molecule; also known as TIMD4), which belongs to the immunoglobulin superfamily and TIM family. TIM4 is involved in regulating Tcell proliferation and lymphotoxin signaling. It is a ligand for HAVCR1/TIMD1. Recent reports indicate that dendritic cell (DC)-derived T-cell immunoglobulin and mucin domain molecule (TIM)-4, which is expressed on dendritic cells and macrophages, plays an important role in the initiation of T(H)2 polarization. TIM4 bound apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain. The expression of TIM4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIM4 is phosphatidylserine receptor for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved. Modulation of TIM4 production in dendritic cells (DCs) represents a novel therapeutic approach for the treatment of peanut allergy. The interaction of TIM1/TIM4 played a critical role in sustaining the polarization status of Th2 cells in allergic rhinitis (AR) patients. Cross-linking FcgammaRI by antigen/IgG complexes increased the production of TIM4 by dendritic cells via upregulating tumor necrosis factoralpha in DCs. Specific immunotherapy (SIT) suppresses the skewed Th2 responses via disrupting the interaction of TIM1/TIM4 in antigen-specific Th2 cells.

NCBI and Uniprot Product Information

• NCBI GI #: 14290446
• NCBI GeneID: 91937
• Molecular Weight: Mol Mass: 32.7 kDa; AP Mol Mass: 60-65 kDa
• NCBI Official Full Name: T-cell immunoglobulin and mucin domain containing 4
• NCBI Official Synonym Full Names: T-cell immunoglobulin and mucin domain containing 4
• NCBI Official Symbol: TIMD4
• NCBI Official Synonym Symbols: TIM4; SMUCKLER
• NCBI Protein Information: T-cell immunoglobulin and mucin domain-containing protein 4
• UniProt Protein Name: T-cell immunoglobulin and mucin domain-containing protein 4
• UniProt Gene Name: TIMD4
• UniProt Synonym Gene Names: TIM4; TIMD-4; TIM-4

Uniprot Description

TIM-4: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3