Tim4/TIMD4 Recombinant Protein | TIMD4 recombinant protein

Tim4/TIMD4 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122715

• Gene Names: Timd4; Tim4; TIM-4; B430010N18Rik
• Purity: >94% as determined by SDS-PAGE
• Synonyms: Tim4/TIMD4; Tim4/TIMD4 Protein; Mouse; Recombinant (His Tag); Mouse TIM4/TIMD4 Protein (His Tag); B430010N18Rik Protein; TIM-4 Protein; Tim4 Protein; T-cell immunoglobulin and mucin domain containing 4; TIMD4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr279

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse TIMD4 (NP_848874.3) precursor (Met 1-Thr 279) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TIMD4 recombinant protein

Background: A type I transmembrane protein called TIM4 (T-cell immunoglobulin- and mucin-domain-containing molecule; also known as TIMD4), which belongs to the immunoglobulin superfamily and TIM family. TIM4 is involved in regulating T-cell proliferation and lymphotoxin signaling. It is a ligand for HAVCR1/TIMD1. Recent reports indicate that dendritic cell (DC)-derived T-cell immunoglobulin and mucin domain molecule (TIM)-4, which is expressed on dendritic cells and macrophages, plays an important role in the initiation of T(H)2 polarization. TIM4 bound apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain. The expression of TIM4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIM4 is phosphatidylserine receptor for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved. Modulation of TIM4 production in dendritic cells (DCs) represents a novel therapeutic approach for the treatment of peanut allergy. The interaction of TIM1/TIM4 played a critical role in sustaining the polarization status of Th2 cells in allergic rhinitis (AR) patients. Cross-linking FcgammaRI by antigen/IgG complexes increased the production of TIM4 by dendritic cells via upregulating tumor necrosis factor-alpha in DCs. Specific immunotherapy (SIT) suppresses the skewed Th2 responses via disrupting the interaction of TIM1/TIM4 in antigen-specific Th2 cells.

References

Miyanishi M, et al. (2007) Identification of Tim4 as a phosphatidylserine receptor. Nature. 450(7168): 435-9.Feng BS, et al. (2008) Disruption of T-cell immunoglobulin and mucin domain molecule (TIM)-1/TIM4 interaction as a therapeutic strategy in a dendritic cell-induced peanut allergy model. J Allergy Clin Immunol. 122(1): 55-61.Cai PC, et al. (2009) Association of TIM4 promoter polymorphism -1419GA with childhood asthma in a Chinese Han population. Tissue Antigens. 74(1): 11-6.Zhao CQ, et al. (2010) Specific immunotherapy suppresses Th2 responses via modulating TIM1/TIM4 interaction on dendritic cells. Allergy. 65(8): 986-95.

NCBI and Uniprot Product Information

• NCBI GI #: 110624776
• NCBI GeneID: 276891
• NCBI Accession #: NP_848874.3
• NCBI GenBank Nucleotide #: NM_178759.4
• UniProt Accession #: Q6U7R4; Q6U7R3; Q8CIC7
• Molecular Weight: 37,548 Da
• NCBI Official Full Name: T-cell immunoglobulin and mucin domain-containing protein 4
• NCBI Official Synonym Full Names: T cell immunoglobulin and mucin domain containing 4
• NCBI Official Symbol: Timd4
• NCBI Official Synonym Symbols: Tim4; TIM-4; B430010N18Rik
• NCBI Protein Information: T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIMD-4; smuckler; spleen, mucin-containing, knockout of lymphotoxin protei
• UniProt Protein Name: T-cell immunoglobulin and mucin domain-containing protein 4
• Protein Family: T-cell immunoglobulin and mucin domain-containing protein
• UniProt Gene Name: Timd4
• UniProt Synonym Gene Names: Tim4; TIMD-4; SMUCKLER; TIM-4
• UniProt Entry Name: TIMD4_MOUSE

Uniprot Description

TIM-4: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane