OX-40L / TNFSF4 / CD252 recombinant protein

Human OX-40L / TNFSF4 / CD252 Protein (Fc Tag)

Cat. Num. AAA2545395

• Gene Names: TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• Purity: > 90 % as determined by SDS-PAGE.
• Synonyms: OX-40L / TNFSF4 / CD252; Human OX-40L / TNFSF4 / CD252 Protein (Fc Tag); TXGP1; OX-40L / TNFSF4 / CD252 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 183

• Application Notes: The recombinant human TNFSF4 consists of 369 amino acids and predicts a molecular mass of 42.1 kDa.
• Predicted N Terminal: Asp
• Endotoxin: <1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for OX-40L / TNFSF4 / CD252 recombinant protein

Background: OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX4. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD40-stimulated B cells, this cytokine along with CD70 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.

Description: A DNA sequence encoding the human TNFSF4 (NP_003317.1) (Gln51-Leu183) was expressed with the Fc region of mouse IgG1 at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4507603
• NCBI GeneID: 7292
• NCBI Accession #: NP_003317.1
• NCBI GenBank Nucleotide #: NM_003326.4
• UniProt Accession #: P23510; Q5JZA5; Q8IV74; Q9HCN9
• Molecular Weight: 15,427 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 4 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 4
• NCBI Official Symbol: TNFSF4
• NCBI Official Synonym Symbols: GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 4
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• UniProt Gene Name: TNFSF4
• UniProt Synonym Gene Names: TXGP1; OX40L

NCBI Description

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TNFSF4: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T-cell-APC interaction and the functional consequences of T-cell activation, thereby destabilizing peripheral tolerance. Belongs to the tumor necrosis factor family.

Protein type: Cell cycle regulation; Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: cell surface; extracellular space; plasma membrane

Molecular Function: receptor binding; tumor necrosis factor receptor superfamily binding

Biological Process: acute inflammatory response; defense response to nematode; negative regulation of interferon-gamma production; negative regulation of interleukin-17 production; negative regulation of regulatory T cell differentiation; negative regulation of T-helper 1 cell differentiation; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of alpha-beta T cell proliferation; positive regulation of B cell activation; positive regulation of CD4-positive, alpha beta T cell differentiation; positive regulation of cell proliferation; positive regulation of immunoglobulin mediated immune response; positive regulation of immunoglobulin secretion; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-13 production; positive regulation of interleukin-4 production; positive regulation of interleukin-6 production; positive regulation of memory T cell differentiation; positive regulation of T cell cytokine production; positive regulation of T cell proliferation; positive regulation of T-helper 2 cell differentiation; positive regulation of T-helper 2 type immune response; regulation of adaptive immune response; regulation of inflammatory response; response to virus; signal transduction; tumor necrosis factor-mediated signaling pathway

Disease: Myocardial Infarction, Susceptibility To