PRC1 recombinant protein

Recombinant Human PRC1 Protein (His tag)

Cat. Num. AAA2545340

• Gene Names: PRC1; ASE1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: PRC1; Recombinant Human PRC1 Protein (His tag); ASE1; PRC1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 20% gly, 3mM DTT
• Sequence Length: 620

• Application Notes: The recombinant human PRC1 consists of 639 amino acids and predicts a molecular mass of 74 kDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for PRC1 recombinant protein

Background: PRC1 (protein regulator of cytokinesis 1) is a key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. It is essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. PRC1 is required for KIF14 localization to the central spindle and midbody. It is also required to recruit PLK1 to the spindle. PRC1 stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. It is a homodimer and interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1. The interaction with RACGAP1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. PRC1 also interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. It interacts with KIF14, IF20A and PLK1.

Description: A DNA sequence encoding the human PRC1 isoform 1 (NP_003972.1) (Met 1-Ser 620) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4506039
• NCBI GeneID: 9055
• NCBI Accession #: NP_003972.1
• NCBI GenBank Nucleotide #: NM_003981.3
• UniProt Accession #: O43663; Q9BSB6; A6NC44; B4DLR1; H9KV59
• Molecular Weight: 70,191 Da
• NCBI Official Full Name: protein regulator of cytokinesis 1 isoform 1
• NCBI Official Synonym Full Names: protein regulator of cytokinesis 1
• NCBI Official Symbol: PRC1
• NCBI Official Synonym Symbols: ASE1
• NCBI Protein Information: protein regulator of cytokinesis 1
• UniProt Protein Name: Protein regulator of cytokinesis 1
• Protein Family: Protein regulator of cytokinesis
• UniProt Gene Name: PRC1

NCBI Description

This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

Uniprot Description

PRC1: translocates to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. Acts as a microtubule-binding and bundling protein both in vivo and vitro. Interacts with the C-terminal Rho-GAP domain and the basic region of MgcRacGAP. The interaction with MgcRacGAP inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis.

Protein type: Cell cycle regulation; Cytoskeletal; Microtubule-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cytoplasm; cytosol; microtubule cytoskeleton; midbody; nucleoplasm; nucleus; plasma membrane; spindle; spindle microtubule

Molecular Function: kinesin binding; protein binding; protein kinase binding

Biological Process: cytokinesis; mitotic spindle elongation; positive regulation of cell proliferation