RAMP3 recombinant protein

Recombinant Human RAMP3 Protein (Fc tag)

Cat. Num. AAA2545254

• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: RAMP3; Recombinant Human RAMP3 Protein (Fc tag); RAMP3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 148

• Application Notes: The recombinant human RAMP3 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 336 amino acids and has a predicted molecular mass of 37.8 kDa. The apparent molecular mass of the protein is approximately 28-31 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Arg 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for RAMP3 recombinant protein

Background: RAMP3 belongs to the RAMP family. Members of this family are single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs have a wide biological distribution; high concentrations are found in the brain, lung, liver, heart and spleen with lower expression levels present in the testes, gastrointestinal tract and thyroid. RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. They are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of RAMP3 protein, CRLR functions as an adrenomedullin receptor.

Description: A DNA sequence encoding the human RAMP3 (Met1-Val118) was expressed with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 5032023
• NCBI GeneID: 10268
• NCBI Accession #: NP_005847.1
• NCBI GenBank Nucleotide #: NM_005856.2
• UniProt Accession #: O60896; Q7Z2Y1
• Molecular Weight: 16,518 Da
• NCBI Official Full Name: receptor activity-modifying protein 3
• NCBI Official Synonym Full Names: receptor activity modifying protein 3
• NCBI Official Symbol: RAMP3
• NCBI Protein Information: receptor activity-modifying protein 3
• UniProt Protein Name: Receptor activity-modifying protein 3
• Protein Family: Receptor activity-modifying protein
• UniProt Gene Name: RAMP3
• UniProt Synonym Gene Names: CRLR activity-modifying protein 3

NCBI Description

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP3) protein, CRLR functions as an adrenomedullin receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

RAMP3: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. Belongs to the RAMP family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: cell surface; integral to plasma membrane; intracellular; lysosome; plasma membrane; receptor complex

Molecular Function: protein binding; protein transporter activity; receptor activity

Biological Process: calcium ion transport; negative regulation of transcription, DNA-dependent; positive regulation of receptor recycling; protein transport; receptor internalization