Rabbit anti-Mouse SPARC  Monoclonal Antibody | anti-SPARC  antibody

Rabbit anti Mouse SPARC  Monoclonal Antibody

Cat. Num. AAA2544593

• Gene Names: SPARC; ON; OI17; BM-40
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation
• Synonyms: SPARC ; Monoclonal Antibody; Rabbit anti Mouse SPARC Monoclonal Antibody; anti-SPARC antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 303

• Applicable Applications for anti-SPARC antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 5-10 mug/mL; ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Mouse Osteonectin/SPARC. The detection limit for Mouse Osteonectin/SPARC is approximately 0.0049 ng/well.; IP: 4-6 mug/mg of lysate
• Immunogen: Recombinant Mouse Osteonectin/SPARC protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-SPARC  antibody

Secreted protein acidic and rich in cysteine (SPARC), also known as Osteonectin (ON), is a member of the SPARC family. SPARC consists of three domains: a EF-hand domain, a follistatin-like domain and a Kazal-like domain, and each of which has independent activity and unique properties. The activity of SPARC is context- and cell-type-dependent, which is highlighted by the fact that SPARC has shown seemingly contradictory effects on tumor progression in both clinical correlative studies and in animal models. The location of SPARC in the nuclear matrix of certain proliferating cells, but only in the cytosol of postmitotic neurons, indicates potential functions of SPARC as a nuclear protein, which might be involved in the regulation of cell cycle progression and mitosis. It functions not only to modulate cell-cell and cell-matrix interactions, but its de-adhesive and growth inhibitory properties in non-transformed cells have led to studies to assess its role in cancer. Its divergent actions reflect the complexity of this protein, because in certain types of cancers, such as melanomas and gliomas, SPARC is associated with a highly aggressive tumor phenotype, while in others, mainly ovarian, neuroblastomas and colorectal cancers, SPARC may function as a tumor suppressor. Recent studies have also demonstrated a role for SPARC in sensitizing therapy-resistant cancers. Notably, SPARC is linked to human obesity.

NCBI and Uniprot Product Information

• NCBI GI #: 4507171
• NCBI GeneID: 6678
• NCBI GenBank Nucleotide #: NM_003118.3
• Molecular Weight: 34,632 Da
• NCBI Official Full Name: SPARC isoform 1
• NCBI Official Synonym Full Names: secreted protein acidic and cysteine rich
• NCBI Official Symbol: SPARC
• NCBI Official Synonym Symbols: ON; OI17; BM-40
• NCBI Protein Information: SPARC
• UniProt Protein Name: SPARC
• UniProt Gene Name: SPARC
• UniProt Synonym Gene Names: ON; BM-40; ON
• UniProt Entry Name: SPRC_HUMAN

NCBI Description

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

Uniprot Description

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; mitochondrion; nuclear matrix; plasma membrane; platelet alpha granule membrane

Molecular Function: calcium ion binding; collagen binding; extracellular matrix binding; protein binding

Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; platelet degranulation; receptor-mediated endocytosis; regulation of cell morphogenesis

Disease: Osteogenesis Imperfecta, Type Xvii