Rabbit anti-Human CES3  Monoclonal Antibody | anti-CES3  antibody

Rabbit anti Human CES3  Monoclonal Antibody

Cat. Num. AAA2544174

• Gene Names: CES3; ES31
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: CES3 ; Monoclonal Antibody; Rabbit anti Human CES3 Monoclonal Antibody; anti-CES3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 210

• Applicable Applications for anti-CES3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 5-10 mug/mL; ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human CES3. The detection limit for Human CES3 is approximately 0.0195 ng/well.
• Immunogen: Recombinant Human CES3/Carboxylesterase 3 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CES3  antibody

Carboxylesterases hydrolyze esters of short-chain fatty acids and have roles in animals ranging from signal transduction to xenobiotic detoxification. In enzymology, a carboxylesterase is an enzyme that catalyzes the chemical reaction: a carboxylic ester + H2O = an alcohol + a carboxylate. Most enzymes from this group belong to the superfamily of hydrolases with alpha/beta protein fold (so called Alpha/beta hydrolase fold), specifically those acting on carboxylic ester bonds. The carboxylesterase family of evolutionarily related proteins (those with clear sequence homology to each other) includes a number of proteins with different substrate specificities, such as acetylcholinesterases. Carboxylesterase 3, also known as Liver carboxylesterase 31 homolog and CES3, is a endoplasmic reticulum lumen which belongs to the type-B carboxylesterase/lipase family. CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES3 shows low catalytic efficiency for hydrolysis of CPT-11, a prodrug for camptothecin used in cancer therapeutics. CES3 is expressed in liver, colon and small intestine.

NCBI and Uniprot Product Information

• NCBI GI #: 297747271
• NCBI GeneID: 23491
• NCBI GenBank Nucleotide #: NM_001185176.1
• Molecular Weight: 24,106 Da
• NCBI Official Full Name: carboxylesterase 3 isoform 3
• NCBI Official Synonym Full Names: carboxylesterase 3
• NCBI Official Symbol: CES3
• NCBI Official Synonym Symbols: ES31
• NCBI Protein Information: carboxylesterase 3
• UniProt Protein Name: Carboxylesterase 3
• UniProt Gene Name: CES3
• UniProt Entry Name: EST3_HUMAN

NCBI Description

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.[provided by RefSeq, Jun 2010]

Uniprot Description

CES3: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows low catalytic efficiency for hydrolysis of CPT-11 (7-ethyl-10-[4-(1-piperidino)- 1-piperidino]-carbonyloxycamptothecin), a prodrug for camptothecin used in cancer therapeutics. Belongs to the type-B carboxylesterase/lipase family.

Protein type: EC 3.1.1.1; Endoplasmic reticulum; Hydrolase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space