Western Blot (WB)
(Western blot analysis of VG5Q expression in HeLa (A) whole cell lysates.)
Rabbit anti-Human VG5Q Polyclonal Antibody | anti-VG5Q antibody
Anti-VG5Q Antibody
IF/ICC: 1:50-1:200
Western Blot (WB)
(Western blot analysis of VG5Q expression in HeLa (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of VG5Q expression in HeLa (A) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of VG5Q staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of VG5Q staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
Uniprot Description
AGGF1: Promotes angiogenesis and the proliferation of endothelial cells. Able to bind to endothelial cells and promote cell proliferation, suggesting that it may act in an autocrine fashion. Defects in AGGF1 are a cause of Klippel-Trenaunay syndrome (KTS). KTS is a congenital disease characterized by malformations of capillary (98% of KTS patients), venous (72%) and lymphatic (11%) vessels, and bony and soft tissue hypertrophy that leads to large cutaneous hemangiomata with hypertrophy of the related bones and soft tissues. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; RNA processing
Chromosomal Location of Human Ortholog: 5q13.3
Cellular Component: cytoplasm; extracellular region; perinuclear region of cytoplasm
Molecular Function: nucleic acid binding; protein binding
Biological Process: angiogenesis; cell adhesion; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; vasculogenesis
Disease: Klippel-trenaunay-weber Syndrome
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Product Notes
The VG5Q aggf1 (Catalog #AAA8305980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VG5Q Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VG5Q can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the VG5Q aggf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VG5Q, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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