Rabbit anti-Human EPHB4  Monoclonal Antibody | anti-EPHB4  antibody

Rabbit anti Human EPHB4  Monoclonal Antibody

Cat. Num. AAA2543891

• Gene Names: EPHB4; HTK; MYK1; TYRO11
• Reactivity: Human
• Applications: ELISA
• Synonyms: EPHB4 ; Monoclonal Antibody; Rabbit anti Human EPHB4 Monoclonal Antibody; anti-EPHB4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 987

• Applicable Applications for anti-EPHB4 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human EPHB4. The detection limit for Human EPHB4 is approximately 0.0049 ng/well.
• Immunogen: Recombinant Human EphB4 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-EPHB4  antibody

Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

NCBI and Uniprot Product Information

• NCBI GI #: 32528301
• NCBI GeneID: 2050
• NCBI GenBank Nucleotide #: NM_004444.4
• Molecular Weight: 45,195 Da
• NCBI Official Full Name: ephrin type-B receptor 4
• NCBI Official Synonym Full Names: EPH receptor B4
• NCBI Official Symbol: EPHB4
• NCBI Official Synonym Symbols: HTK; MYK1; TYRO11
• NCBI Protein Information: ephrin type-B receptor 4
• UniProt Protein Name: Ephrin type-B receptor 4
• UniProt Gene Name: EPHB4
• UniProt Synonym Gene Names: HTK; MYK1; TYRO11
• UniProt Entry Name: EPHB4_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008]

Uniprot Description

EphB4: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: cytosol; extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: ephrin receptor activity; protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: angiogenesis; cell adhesion; cell migration during sprouting angiogenesis; ephrin receptor signaling pathway; heart morphogenesis; protein amino acid autophosphorylation