Mouse anti-Human ADAM12/MLTN Monoclonal Antibody | anti-ADAM12/MLTN antibody

Mouse anti Human ADAM12/MLTN Monoclonal Antibody

Cat. Num. AAA2543505

• Gene Names: ADAM12; MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
• Reactivity: Human
• Applications: ELISA
• Synonyms: ADAM12/MLTN; Monoclonal Antibody; Mouse anti Human ADAM12/MLTN Monoclonal Antibody; anti-ADAM12/MLTN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Sequence Length: 737

• Applicable Applications for anti-ADAM12/MLTN antibody: ELISA (EIA)
• Application Notes: ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect Human ADAM12. The detection limit for Human ADAM12 is 0.078 ng/well.
• Immunogen: Recombinant Human ADAM12 protein
• Buffer: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ADAM12/MLTN extracellular domain (rh ADAM12; NP_003465.3; Met 1-Ser 513). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 38173792
• NCBI GeneID: 8038
• Molecular Weight: 80,263 Da
• NCBI Official Full Name: ADAM metallopeptidase domain 12
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 12
• NCBI Official Symbol: ADAM12
• NCBI Official Synonym Symbols: MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 12
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 12
• UniProt Gene Name: ADAM12
• UniProt Synonym Gene Names: MLTN; ADAM 12
• UniProt Entry Name: ADA12_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]

Uniprot Description

ADAM12: contains a disintegrin and metalloprotease (ADAM) domain 12, which is a member of the ADAM protein family. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion, and the differentiation of macrophage-derived giant cells (MGC) and osteoclasts. 2 alternative splicing isoforms have been described: a shorter secreted form and a longer membrane-bound form. The shorter form stimulates myogenesis.

Protein type: EC 3.4.24.-; Membrane protein, integral; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: mitochondrion; nucleoplasm; plasma membrane

Molecular Function: metallopeptidase activity; SH3 domain binding

Biological Process: myoblast fusion