Mouse EEF1D Monoclonal Antibody | anti-EEF1D antibody

EEF1D Monoclonal Antibody

Cat. Num. AAA2541902

• Gene Names: EEF1D; EF1D; EF-1D; FP1047
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Caprylic acid/ammonium sulfate precipitation
• Synonyms: EEF1D; Monoclonal Antibody; EEF1D Monoclonal Antibody; Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; Elongation factor 1 delta; Elongation factor 1-delta; eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein); Eukaryotic translation elongation factor 1 delta; FP1047; Guanine nucleotide exchange protein; anti-EEF1D antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 98
• Purity/Purification: Caprylic acid/ammonium sulfate precipitation
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 647

• Applicable Applications for anti-EEF1D antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200
• Immunogen: Fusion protein of EEF1D
• Buffer: PBS with 0.1% sodium azide, 50% glycerol, PH7.3
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of Human pancreas cancer using EEF1D Monoclonal antibody at dilution of 1:50)

Related Product Information for anti-EEF1D antibody

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human EEF1D.

Product Categories/Family for anti-EEF1D antibody

Epigenetics and Nuclear Signaling; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 304555583
• NCBI GeneID: 1936
• NCBI Accession #: NP_001123525.2
• NCBI GenBank Nucleotide #: NM_001130053.3
• UniProt Accession #: P29692; Q4VBZ6; Q969J1; Q96I38; B4DDU4; D3DWK3; E9PBQ9
• Molecular Weight: Calculated: 71kDa; Observed: 31kDa; 72kDa
• NCBI Official Full Name: elongation factor 1-delta isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 delta
• NCBI Official Symbol: EEF1D
• NCBI Official Synonym Symbols: EF1D; EF-1D; FP1047
• NCBI Protein Information: elongation factor 1-delta
• UniProt Protein Name: Elongation factor 1-delta
• Protein Family: Elongation factor
• UniProt Gene Name: EEF1D
• UniProt Synonym Gene Names: EF1D; EF-1-delta

NCBI Description

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]

Uniprot Description

Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.