Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using ERCC2 antibody. Blue: DAPI for nuclear staining.)
Rabbit ERCC2 Polyclonal Antibody | anti-ERCC2 antibody
ERCC2 Polyclonal Antibody
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using ERCC2 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cell using ERCC2 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Uniprot Description
ERCC2: ATP-dependent 5'-3' DNA helicase, component of the core- TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. Might also have a role in aging process and could play a causative role in the generation of skin cancers. Belongs to the helicase family. RAD3/XPD subfamily. One of the six subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. The interaction with GTF2H2 results in the stimulation of the 5'-->3' helicase activity. Interacts with Epstein-Barr virus EBNA2.
Protein type: Helicase; EC 3.6.4.12
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: cyclin-dependent protein kinase activating kinase holoenzyme complex; cytoplasm; holo TFIIH complex; nucleoplasm; nucleus; spindle
Molecular Function: 4 iron, 4 sulfur cluster binding; 5'-3' DNA helicase activity; ATP binding; ATP-dependent DNA helicase activity; DNA binding; DNA-dependent ATPase activity; metal ion binding; protein binding; protein C-terminus binding; protein kinase activity; protein N-terminus binding; RNA polymerase subunit kinase activity
Biological Process: aging; apoptosis; bone mineralization; cell proliferation; chromosome segregation; DNA duplex unwinding; DNA repair; embryonic cleavage; erythrocyte maturation; extracellular matrix organization and biogenesis; gene expression; hair cell differentiation; hair follicle maturation; in utero embryonic development; mRNA capping; multicellular organism growth; myelin formation in the central nervous system; negative regulation of gene expression, epigenetic; nucleotide-excision repair; nucleotide-excision repair, DNA incision; positive regulation of DNA binding; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of viral transcription; post-embryonic development; protein amino acid phosphorylation; regulation of gene expression, epigenetic; response to hypoxia; response to oxidative stress; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; spinal cord development; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; UV protection; viral reproduction
Disease: Cerebrooculofacioskeletal Syndrome 2; Trichothiodystrophy, Photosensitive; Xeroderma Pigmentosum, Complementation Group D
Research Articles on ERCC2
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Product Notes
The ERCC2 ercc2 (Catalog #AAA2535775) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERCC2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-100. Researchers should empirically determine the suitability of the ERCC2 ercc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERCC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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