Human IRAK3 (Interleukin 1 Receptor Associated Kinase 3) CLIA Kit | IRAK3 clia kit

Human IRAK3 (Interleukin 1 Receptor Associated Kinase 3) CLIA Kit

Cat. Num. AAA2533095

• Gene Names: IRAK3; ASRT5; IRAKM
• Reactivity: Human
• Synonyms: IRAK3 (Interleukin 1 Receptor Associated Kinase 3); Human IRAK3 (Interleukin 1 Receptor Associated Kinase 3) CLIA Kit; IRAK3 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant IRAK3. No significant cross-reactivity or interference between IRAK3 and analogues was observed.
• Sequence Length: 596

• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRAK3 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of IRAK3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to IRAK3. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IRAK3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IRAK3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of IRAK3. You can calculate the concentration of IRAK3 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119617570
• NCBI GeneID: 11213
• Molecular Weight: 60,608 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 3
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 3
• NCBI Official Symbol: IRAK3
• NCBI Official Synonym Symbols: ASRT5; IRAKM
• NCBI Protein Information: interleukin-1 receptor-associated kinase 3
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 3
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK3
• UniProt Synonym Gene Names: IRAK-3; IRAK-M
• UniProt Entry Name: IRAK3_HUMAN

NCBI Description

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

IRAK3: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; magnesium ion binding; protein heterodimerization activity; protein homodimerization activity; protein serine/threonine kinase activity

Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inhibition of NF-kappaB transcription factor; MyD88-dependent toll-like receptor signaling pathway; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of innate immune response; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of MAP kinase activity; negative regulation of protein catabolic process; negative regulation of protein complex disassembly; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of protein complex disassembly; response to exogenous dsRNA; response to lipopolysaccharide; response to peptidoglycan; response to virus

Disease: Asthma-related Traits, Susceptibility To, 5