Human IRAK3 ELISA Kit | IRAK3 elisa kit

Human IRAK3 (Interleukin 1 Receptor Associated Kinase 3) ELISA Kit

Cat. Num. AAA2509721

• Gene Names: IRAK3; ASRT5; IRAKM
• Reactivity: Human
• Synonyms: IRAK3; Human IRAK3 (Interleukin 1 Receptor Associated Kinase 3) ELISA Kit; IRAK3 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human IRAK3. No significant cross-reactivity or interference between Human IRAK3 and analogues was observed.
• Sequence Length: 596

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRAK3 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IRAK3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IRAK3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IRAK3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IRAK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IRAK3. You can calculate the concentration of IRAK3 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 216547519
• NCBI GeneID: 11213
• NCBI Accession #: NP_009130.2
• NCBI GenBank Nucleotide #: NM_007199.2
• UniProt Accession #: Q9Y616; B4DQ57
• Molecular Weight: 60,608 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 3 isoform a
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 3
• NCBI Official Symbol: IRAK3
• NCBI Official Synonym Symbols: ASRT5; IRAKM
• NCBI Protein Information: interleukin-1 receptor-associated kinase 3; IL-1 receptor-associated kinase M; IRAK3
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 3
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK3
• UniProt Synonym Gene Names: IRAK-3; IRAK-M
• UniProt Entry Name: IRAK3_HUMAN

NCBI Description

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

IRAK3: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, TKL; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: interleukin-1 receptor complex; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; ATP binding

Biological Process: negative regulation of MAP kinase activity; regulation of protein complex disassembly; negative regulation of interleukin-12 production; response to virus; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; MyD88-dependent toll-like receptor signaling pathway; response to exogenous dsRNA; inhibition of NF-kappaB transcription factor; negative regulation of innate immune response; response to peptidoglycan; toll-like receptor signaling pathway; negative regulation of interleukin-6 production; negative regulation of toll-like receptor signaling pathway; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of protein catabolic process; negative regulation of protein complex disassembly

Disease: Asthma-related Traits, Susceptibility To, 5