Human CX40 (Connexin 40) CLIA Kit | CX40 clia kit

Human CX40 (Connexin 40) CLIA Kit

Cat. Num. AAA2532383

• Gene Names: GJA5; CX40; ATFB11
• Reactivity: Human
• Synonyms: CX40 (Connexin 40); Human CX40 (Connexin 40) CLIA Kit; CX40 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant CX40. No significant cross-reactivity or interference between CX40 and analogues was observed.
• Sequence Length: 358

• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CX40 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of CX40 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to CX40. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CX40 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CX40, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of CX40. You can calculate the concentration of CX40 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1220303
• NCBI GeneID: 2702
• Molecular Weight: 40,380 Da
• NCBI Official Full Name: connexin 40
• NCBI Official Synonym Full Names: gap junction protein, alpha 5, 40kDa
• NCBI Official Symbol: GJA5
• NCBI Official Synonym Symbols: CX40; ATFB11
• NCBI Protein Information: gap junction alpha-5 protein
• UniProt Protein Name: Gap junction alpha-5 protein
• UniProt Gene Name: GJA5
• UniProt Synonym Gene Names: Cx40
• UniProt Entry Name: CXA5_HUMAN

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

GJA5: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJA5 are a cause of familial atrial standstill (FAS). Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. Defects in GJA5 are the cause of familial atrial fibrillation type 11 (ATFB11). ATFB11 is a familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; Channel, misc.

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: connexon complex; cell projection; integral to plasma membrane; gap junction

Molecular Function: gap junction hemi-channel activity

Biological Process: negative regulation of glomerular filtration; gap junction assembly; positive regulation of vasodilation; protein oligomerization; positive regulation of vasoconstriction; artery morphogenesis; embryonic heart tube development; negative regulation of blood pressure; angiogenesis; transmembrane transport; skeletal development; potassium ion transport; embryonic limb morphogenesis

Disease: Atrial Standstill 1; Atrial Fibrillation, Familial, 11