Typical Testing Data/Standard Curve (for reference only)
Mouse IL-2R (Interleukin-2 Receptor) CLIA Kit | IL-2R clia kit
Mouse IL-2R (Interleukin-2 Receptor) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to IL-2R. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-2R and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-2R, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of IL-2R. You can calculate the concentration of IL-2R in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Uniprot Description
IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 10p15-p14
Cellular Component: plasma membrane; integral to membrane; intracellular; external side of plasma membrane
Molecular Function: interleukin-2 receptor activity; drug binding; interleukin-2 binding
Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; regulation of T cell homeostatic proliferation; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; apoptosis; negative regulation of immune response; MAPKKK cascade; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; cell proliferation; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; inflammatory response to antigenic stimulus; positive regulation of T cell differentiation; small GTPase mediated signal transduction; negative regulation of inflammatory response; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; immune response; inflammatory response; vascular endothelial growth factor receptor signaling pathway; activated T cell apoptosis
Disease: Interleukin 2 Receptor, Alpha, Deficiency Of; Diabetes Mellitus, Insulin-dependent, 10
Research Articles on IL-2R
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Product Notes
The Mouse IL-2R il2ra (Catalog #AAA2532124) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2532124 CLIA Kit recognizes Mouse IL-2R. It is sometimes possible for the material contained within the vial of "IL-2R (Interleukin-2 Receptor), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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