Typical Testing Data/Standard Curve (for reference only)
Mouse PDGFB (Platelet Derived Growth Factor Subunit B) CLIA Kit | PDGFB clia kit
Mouse PDGFB (Platelet Derived Growth Factor Subunit B) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PDGFB. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDGFB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDGFB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PDGFB. You can calculate the concentration of PDGFB in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013]
Uniprot Description
PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family.
Protein type: Secreted, signal peptide; Secreted; Oncoprotein; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: basolateral plasma membrane; cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi membrane
Molecular Function: chemoattractant activity; collagen binding; growth factor activity; identical protein binding; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; superoxide-generating NADPH oxidase activator activity
Biological Process: activation of MAPKK activity; activation of protein kinase activity; activation of protein kinase B; axon guidance; blood coagulation; cell growth; DNA replication; embryonic placenta development; epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; fibroblast growth factor receptor signaling pathway; heart development; hemopoiesis; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; monocyte chemotaxis; negative regulation of phosphatidylinositol biosynthetic process; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; platelet activation; platelet degranulation; platelet-derived growth factor receptor signaling pathway; positive chemotaxis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of cyclin-dependent protein kinase activity; positive regulation of DNA replication; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of glomerular filtration; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; Ras protein signal transduction; response to drug; response to estradiol stimulus; response to hypoxia; response to wounding; small GTPase mediated signal transduction; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway
Disease: Basal Ganglia Calcification, Idiopathic, 1; Basal Ganglia Calcification, Idiopathic, 5; Dermatofibrosarcoma Protuberans; Meningioma, Familial, Susceptibility To
Research Articles on PDGFB
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Product Notes
The Mouse PDGFB pdgfb (Catalog #AAA2530991) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2530991 CLIA Kit recognizes Mouse PDGFB. It is sometimes possible for the material contained within the vial of "PDGFB (Platelet Derived Growth Factor Subunit B), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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