Mouse NFkB (Nuclear Factor Kappa B) CLIA Kit | NFkB clia kit
Mouse NFkB (Nuclear Factor Kappa B) CLIA Kit
Reactivity
Mouse
Synonyms
NFkB (Nuclear Factor Kappa B); Mouse NFkB (Nuclear Factor Kappa B) CLIA Kit; NFKB1; NF-KB1; NF-Kappa-B; NFKB-p105; NFKB-p50; p105; p50; EBP-1; KBF1; Mouse NFkB; NFkB clia kit
Reactivity
Mouse
Specificity
This kit recognizes natural and recombinant NFkappaB. No significant cross-reactivity or interference between NFkappaB and analogues was observed.
Sequence Length
1095
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Related Product Information for NFkB clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of NFkappaB concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to NFkappaB. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NFkappaB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NFkappaB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of NFkappaB. You can calculate the concentration of NFkappaB in the samples by comparing the RLU value of the samples to the standard curve.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to NFkappaB. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NFkappaB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NFkappaB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of NFkappaB. You can calculate the concentration of NFkappaB in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
nuclear factor kappa B
NCBI Official Symbol
Nfkb
NCBI Protein Information
nuclear factor kappa B
