Rabbit p38 Polyclonal Antibody | anti-CSE antibody

p38 Antibody: ATTO 488

Cat. Num. AAA804954

• Gene Names: CSE; DYT9
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Rabbit, Pig, Canine, Hamster, Chicken, Sheep, Guinea pig
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: p38; Polyclonal Antibody; p38 Antibody: ATTO 488; CSAID Binding protein 1; CSBP1; CSBP2; EXIP; MAP kinase MXI2; MAPkinase p38alpha; MAPK14; p38 ALPHA; p38 MAP kinase; p38 mitogen activated protein kinase; RK; SAPK 2A; Stress activated protein kinase 2A; anti-CSE antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Rabbit, Pig, Canine, Hamster, Chicken, Sheep, Guinea pig
• Clonality: Polyclonal
• Form/Format: Rabbit Antiserum
• Sequence Length: 153

• Applicable Applications for anti-CSE antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: 1:5000 (ECL) (WB), 1:250 (IP)
• Conjugate: ATTO 488
• Storage Buffer: Rabbit Antiserum
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human cancer cell lines showing detection of p38 protein using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Related Product Information for anti-CSE antibody

Background Info: Detects a ~43kDa protein corresponding to the molecular mass of p38 on SDS PAGE immunoblots.

Scientific Background: The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2alpha translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).

Product Categories/Family for anti-CSE antibody

Cell Signaling

References

1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946-1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899- 907. 7. Medicherla S. et al. (2006). J Pharmacol Exp Ther. 318(1): 99-107.

NCBI and Uniprot Product Information

• NCBI GeneID: 1433
• UniProt Accession #: Q16540; Q96Q71; A8MT29
• Molecular Weight: 17,781 Da
• NCBI Official Synonym Full Names: choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity)
• NCBI Official Symbol: CSE
• NCBI Official Synonym Symbols: DYT9
• UniProt Protein Name: 39S ribosomal protein L23, mitochondrial
• Protein Family: Caffeoylshikimate esterase
• UniProt Gene Name: MRPL23
• UniProt Synonym Gene Names: L23MRP; RPL23L; L23mt; MRP-L23
• UniProt Entry Name: RM23_HUMAN

Uniprot Description

MRPL23: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial; Ribosomal; RNA-binding

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: mitochondrion; mitochondrial inner membrane; nucleolus; mitochondrial large ribosomal subunit

Molecular Function: structural constituent of ribosome; RNA binding; nucleotide binding

Biological Process: mitochondrial translation; translation; organelle organization and biogenesis