Typical Testing Data/Standard Curve (for reference only)
Human SDH (Sorbitol Dehydrogenase) CLIA Kit | SDH clia kit
Human SDH (Sorbitol Dehydrogenase) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to SDH. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SDH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SDH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of SDH. You can calculate the concentration of SDH in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
Uniprot Description
SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.
Protein type: Carbohydrate Metabolism - fructose and mannose; EC 1.1.1.14; Oxidoreductase
Chromosomal Location of Human Ortholog: 15q15.3
Cellular Component: extracellular space; membrane; mitochondrial membrane; cytosol
Molecular Function: identical protein binding; zinc ion binding; L-iditol 2-dehydrogenase activity; carbohydrate binding; NAD binding
Biological Process: response to drug; response to nutrient levels; L-xylitol metabolic process; response to hormone stimulus; glucose metabolic process; response to osmotic stress; sorbitol catabolic process; sperm motility; response to cadmium ion; response to copper ion; carbohydrate metabolic process; L-xylitol catabolic process; fructose biosynthetic process; fructose metabolic process
Research Articles on SDH
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Product Notes
The Human SDH sord (Catalog #AAA2530447) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2530447 CLIA Kit recognizes Human SDH. It is sometimes possible for the material contained within the vial of "SDH (Sorbitol Dehydrogenase), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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