Rabbit TCF4 Polyclonal Antibody | anti-TCF4 antibody

TCF4 Antibody

Cat. Num. AAA2520517

• Gene Names: TCF4; E2-2; ITF2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antigen affinity purification
• Synonyms: TCF4; Polyclonal Antibody; TCF4 Antibody; E2-2; ITF2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; bHLHb19; anti-TCF4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 773

• Applicable Applications for anti-TCF4 antibody: Western Blot (WB)
• Immunogen: Recombinant protein of human TCF4
• Observed Molecular Weight: 71kDa
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-48947 / sc-48949 / sc-48950 / sc-48948
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TCF4 antibody.)

Related Product Information for anti-TCF4 antibody

LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for beta-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4, 5).

NCBI and Uniprot Product Information

• NCBI GI #: 597709768
• NCBI GeneID: 6925
• NCBI Accession #: NP_001230155.2
• NCBI GenBank Nucleotide #: NM_001243226.2
• Molecular Weight: 48,098 Da
• NCBI Official Full Name: transcription factor 4 isoform c
• NCBI Official Synonym Full Names: transcription factor 4
• NCBI Official Symbol: TCF4
• NCBI Official Synonym Symbols: E2-2; ITF2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
• NCBI Protein Information: transcription factor 4; SL3-3 enhancer factor 2; class B basic helix-loop-helix protein 19; immunoglobulin transcription factor 2; transcription factor 4, isoform C; transcription factor 4, isoform D; transcription factor 4, isoform E; transcription factor 4, isoform R
• UniProt Protein Name: Transcription factor 4
• Protein Family: Transcription factor
• UniProt Gene Name: TCF4
• UniProt Synonym Gene Names: BHLHB19; ITF2; SEF2; TCF-4; bHLHb19; ITF-2; SEF-2
• UniProt Entry Name: ITF2_HUMAN

NCBI Description

This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Aug 2011]

Uniprot Description

TCF4: Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2. Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: transcription factor complex; nucleus

Molecular Function: protein C-terminus binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription initiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; protein-DNA complex assembly

Disease: Corneal Dystrophy, Fuchs Endothelial, 3; Pitt-hopkins Syndrome