Human FGF8 ELISA Kit | FGF8 elisa kit

Human FGF8 (Fibroblast Growth Factor 8)ELISA Kit

Cat. Num. AAA2516298

• Gene Names: FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8
• Reactivity: Human
• Synonyms: FGF8; Human FGF8 (Fibroblast Growth Factor 8)ELISA Kit; FGF8 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human Fcgamma in samples. No significant cross-reactivity or interference between Human Fcgamma and analogues was observed.
• Sequence Length: 140

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Fcgamma were tested 20 times on one plate, respectively
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Fcgamma were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF8 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human Fcgamma concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Fcgamma. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Fcgamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Fcgamma, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human Fcgamma. You can calculate the concentration of Human Fcgamma in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 329755303
• NCBI GeneID: 2253
• NCBI Accession #: NP_001193318.1
• NCBI GenBank Nucleotide #: NM_001206389.1
• Molecular Weight: 27,715 Da
• NCBI Official Full Name: fibroblast growth factor 8 isoform G
• NCBI Official Synonym Full Names: fibroblast growth factor 8 (androgen-induced)
• NCBI Official Symbol: FGF8
• NCBI Official Synonym Symbols: HH6; AIGF; KAL6; FGF-8; HBGF-8
• NCBI Protein Information: fibroblast growth factor 8; androgen-induced growth factor; heparin-binding growth factor 8
• UniProt Protein Name: Fibroblast growth factor 8
• UniProt Gene Name: FGF8
• UniProt Synonym Gene Names: AIGF; FGF-8; AIGF; HBGF-8
• UniProt Entry Name: FGF8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF8: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone- synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous. Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: growth factor activity; type 2 fibroblast growth factor receptor binding; type 1 fibroblast growth factor receptor binding; fibroblast growth factor receptor binding; chemoattractant activity

Biological Process: gonad development; nerve growth factor receptor signaling pathway; apoptosis; cell proliferation in forebrain; adrenocorticotropin hormone secreting cell differentiation; gastrulation; thyroid stimulating hormone secreting cell differentiation; forebrain dorsal/ventral pattern formation; motor axon guidance; Wnt receptor signaling pathway through beta-catenin; mesodermal cell migration; response to organic cyclic substance; embryonic hindlimb morphogenesis; odontogenesis; BMP signaling pathway; positive chemotaxis; induction of an organ; positive regulation of cell proliferation; thyroid gland development; male genitalia development; pallium development; mesonephros development; negative regulation of neuron apoptosis; heart looping; otic vesicle formation; regulation of odontogenesis of dentine-containing teeth; negative regulation of cardiac muscle development; response to drug; epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; phosphoinositide-mediated signaling; pharyngeal system development; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; neural plate morphogenesis; MAPKKK cascade; subpallium development; forebrain morphogenesis; positive regulation of organ growth; dorsal/ventral axon guidance; patterning of blood vessels; forebrain neuron development; ureteric bud branching; midbrain-hindbrain boundary development; insulin receptor signaling pathway; innate immune response; blood vessel remodeling; response to oxidative stress; metanephros development

Disease: Hypogonadotropic Hypogonadism 6 With Or Without Anosmia