Human PIBF ELISA Kit | PIBF elisa kit

Human PIBF (Progesterone-induced-blocking factor) ELISA Kit

Cat. Num. AAA2516289

• Gene Names: PIBF1; PIBF; CEP90; C13orf24
• Reactivity: Human
• Synonyms: PIBF; Human PIBF (Progesterone-induced-blocking factor) ELISA Kit; PIBF elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human PIBF in samples. No significant cross-reactivity or interference between Human PIBF and analogues was observed.
• Sequence Length: 757

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PIBF were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PIBF were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PIBF elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PIBF concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIBF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PIBF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIBF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PIBF. You can calculate the concentration of Human PIBF in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55769583
• NCBI GeneID: 10464
• NCBI Accession #: NP_006337.2
• NCBI GenBank Nucleotide #: NM_006346.2
• Molecular Weight: 12,016 Da
• NCBI Official Full Name: progesterone-induced-blocking factor 1
• NCBI Official Synonym Full Names: progesterone immunomodulatory binding factor 1
• NCBI Official Symbol: PIBF1
• NCBI Official Synonym Symbols: PIBF; CEP90; C13orf24
• NCBI Protein Information: progesterone-induced-blocking factor 1; progesterone-induced blocking factor 1
• UniProt Protein Name: Progesterone-induced-blocking factor 1
• UniProt Gene Name: PIBF1
• UniProt Synonym Gene Names: C13orf24; PIBF
• UniProt Entry Name: PIBF1_HUMAN

Uniprot Description

PIBF1: Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 13q22.1

Cellular Component: centrosome

Molecular Function: protein binding