Mouse FNDC5 ELISA Kit | FNDC5 elisa kit

Mouse FNDC5(Fibronectin type III domain-containing protein 5) ELISA Kit

Cat. Num. AAA2515765

• Gene Names: FNDC5; FRCP2; irisin
• Reactivity: Mouse
• Synonyms: FNDC5; Mouse FNDC5(Fibronectin type III domain-containing protein 5) ELISA Kit; FNDC5 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes Mouse FNDC5 in samples. No significant cross-reactivity or interference between Mouse FNDC5 and analogues was observed.
• Sequence Length: 212

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 37.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse FNDC5 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse FNDC5 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FNDC5 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse FNDC5 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FNDC5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FNDC5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FNDC5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse FNDC5. You can calculate the concentration of Mouse FNDC5 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 511094000
• NCBI GeneID: 252995
• NCBI Accession #: NP_715637.2
• NCBI GenBank Nucleotide #: NM_153756.2
• Molecular Weight: 15,527 Da
• NCBI Official Full Name: fibronectin type III domain-containing protein 5 isoform 2 preproprotein
• NCBI Official Synonym Full Names: fibronectin type III domain containing 5
• NCBI Official Symbol: FNDC5
• NCBI Official Synonym Symbols: FRCP2; irisin
• NCBI Protein Information: fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2
• UniProt Protein Name: Fibronectin type III domain-containing protein 5
• Protein Family: Fibronectin type III domain-containing protein
• UniProt Gene Name: FNDC5
• UniProt Synonym Gene Names: FRCP2
• UniProt Entry Name: FNDC5_HUMAN

NCBI Description

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: peroxisomal membrane; endoplasmic reticulum; integral to membrane; plasma membrane; extracellular region

Molecular Function: hormone activity

Biological Process: response to muscle activity