Typical Testing Data/Standard Curve (for reference only)
Human GDN ELISA Kit | GDN elisa kit
Human GDN(Glia Derived Nexin)ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GDN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GDNand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GDN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GDN.You can calculate the concentration of GDNin the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. Thrombin, urokinase, plasmin and trypsin are among the proteases that this family member can inhibit. This gene is a susceptibility gene for chronic obstructive pulmonary disease and for emphysema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
SERPINE2: Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 2q36.1
Cellular Component: extracellular matrix; extracellular space; extrinsic to external side of plasma membrane; extracellular region; cytosol; neuromuscular junction; vesicle
Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; protein binding; glycosaminoglycan binding; receptor binding
Biological Process: negative regulation of proteolysis; regulation of synaptic transmission, glutamatergic; secretory granule organization and biogenesis; cerebellar granular layer morphogenesis; negative regulation of blood coagulation; mating plug formation; detection of mechanical stimulus involved in sensory perception; regulation of cell migration; negative regulation of cell proliferation; negative regulation of smoothened signaling pathway; negative regulation of cell growth; blood coagulation; negative regulation of protein catabolic process; regulation of timing of cell differentiation; negative regulation of phosphoinositide 3-kinase cascade; secretion by cell; positive regulation of astrocyte differentiation
Research Articles on GDN
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Product Notes
The Human GDN serpine2 (Catalog #AAA2515745) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2515745 ELISA Kit recognizes Human GDN. It is sometimes possible for the material contained within the vial of "GDN, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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