Human Glia Derived Nexin (GDN) ELISA Kit | GDN elisa kit

Human Glia Derived Nexin (GDN) ELISA Kit

Cat. Num. AAA2024796

• Gene Names: SERPINE2; GDN; PI7; PN1; PNI; PI-7; PN-1; GDNPF
• Reactivity: Human
• Synonyms: Glia Derived Nexin (GDN); Human Glia Derived Nexin (GDN) ELISA Kit; SERPINE2; PI7; PN1; PNI; Protease Nexin I; Serpin Peptidase Inhibitor Clade E Member 2; Peptidase inhibitor 7; Protease nexin I; GDN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glia Derived Nexin (GDN).; No significant cross-reactivity or interference between Glia Derived Nexin (GDN) and analogues was observed.
• Sequence Length: 409

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glia Derived Nexin (GDN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glia Derived Nexin (GDN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glia Derived Nexin (GDN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glia Derived Nexin (GDN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glia Derived Nexin (GDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glia Derived Nexin (GDN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GDN elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 211904156
• NCBI GeneID: 5270
• NCBI Accession #: NP_001130002.1
• NCBI GenBank Nucleotide #: NM_001136530.1
• Molecular Weight: 45,267 Da
• NCBI Official Full Name: glia-derived nexin isoform c
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• NCBI Official Symbol: SERPINE2
• NCBI Official Synonym Symbols: GDN; PI7; PN1; PNI; PI-7; PN-1; GDNPF
• NCBI Protein Information: glia-derived nexin
• UniProt Protein Name: Glia-derived nexin
• UniProt Gene Name: SERPINE2
• UniProt Synonym Gene Names: PI7; PN1; GDN; PI-7; PN-1
• UniProt Entry Name: GDN_HUMAN

NCBI Description

This gene encodes a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. Thrombin, urokinase, plasmin and trypsin are among the proteases that this family member can inhibit. This gene is a susceptibility gene for chronic obstructive pulmonary disease and for emphysema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

SERPINE2: Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: extracellular matrix; extracellular space; extrinsic to external side of plasma membrane; extracellular region; cytosol; neuromuscular junction; vesicle

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; protein binding; glycosaminoglycan binding; receptor binding

Biological Process: negative regulation of proteolysis; regulation of synaptic transmission, glutamatergic; secretory granule organization and biogenesis; cerebellar granular layer morphogenesis; negative regulation of blood coagulation; detection of mechanical stimulus involved in sensory perception; mating plug formation; regulation of cell migration; negative regulation of cell proliferation; negative regulation of smoothened signaling pathway; negative regulation of cell growth; blood coagulation; negative regulation of protein catabolic process; regulation of timing of cell differentiation; positive regulation of astrocyte differentiation; secretion by cell; negative regulation of phosphoinositide 3-kinase cascade