Typical Testing Data/Standard Curve (for reference only)
Human ADAM9 ELISA Kit | ADAM9 elisa kit
Human ADAM9 (A Disintegrin And Metalloprotease 9) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADAM9. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ADAM9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAM9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ADAM9. You can calculate the concentration of Human ADAM9 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
ADAM9: Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.4.24.-
Chromosomal Location of Human Ortholog: 8p11.22
Cellular Component: extracellular space; cell surface; focal adhesion; basolateral plasma membrane; cytoplasm; integral to membrane
Molecular Function: integrin binding; collagen binding; protein binding; protein kinase C binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; laminin binding; SH3 domain binding
Biological Process: positive regulation of keratinocyte migration; integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; activation of MAPKK activity; cell-matrix adhesion; membrane protein ectodomain proteolysis; response to glucocorticoid stimulus; positive regulation of cell adhesion mediated by integrin; positive regulation of membrane protein ectodomain proteolysis; keratinocyte differentiation; response to manganese ion; extracellular matrix disassembly; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; response to hydrogen peroxide; positive regulation of protein secretion; monocyte activation; transforming growth factor beta receptor signaling pathway; cell-cell adhesion mediated by integrin; cell adhesion mediated by integrin; cell adhesion; response to calcium ion
Disease: Cone-rod Dystrophy 9
Research Articles on ADAM9
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Product Notes
The Human ADAM9 adam9 (Catalog #AAA2514811) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2514811 ELISA Kit recognizes Human ADAM9. It is sometimes possible for the material contained within the vial of "ADAM9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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