Mouse Arginine Vasopressin Receptor 2 (AVPR2) ELISA Kit | AVPR2 elisa kit

Mouse Arginine Vasopressin Receptor 2 (AVPR2) ELISA Kit

Cat. Num. AAA2021966

• Gene Names: AVPR2; DI1; DIR; NDI; V2R; ADHR; DIR3
• Reactivity: Mouse
• Synonyms: Arginine Vasopressin Receptor 2 (AVPR2); Mouse Arginine Vasopressin Receptor 2 (AVPR2) ELISA Kit; ADHR; DI1; DIR; DIR3; NDI; V2R; Nephrogenic Diabetes Insipidus; Vasopressin V2 receptor; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor; AVPR2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arginine Vasopressin Receptor 2 (AVPR2).; No significant cross-reactivity or interference between Arginine Vasopressin Receptor 2 (AVPR2) and analogues was observed.
• Sequence Length: 309

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginine Vasopressin Receptor 2 (AVPR2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginine Vasopressin Receptor 2 (AVPR2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AVPR2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin Receptor 2 (AVPR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginine Vasopressin Receptor 2 (AVPR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginine Vasopressin Receptor 2 (AVPR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arginine Vasopressin Receptor 2 (AVPR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AVPR2 elisa kit

Signal transduction; Endocrinology; Cardiovascular biology; Kidney biomarker; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 29825389
• NCBI GeneID: 554
• UniProt Accession #: P30518; O43192; Q3MJD3; Q9UCV9; C5HF20
• Molecular Weight: 33,743 Da
• NCBI Official Full Name: arginine vasopressin receptor 2
• NCBI Official Synonym Full Names: arginine vasopressin receptor 2
• NCBI Official Symbol: AVPR2
• NCBI Official Synonym Symbols: DI1; DIR; NDI; V2R; ADHR; DIR3
• NCBI Protein Information: vasopressin V2 receptor
• UniProt Protein Name: Vasopressin V2 receptor
• Protein Family: Vasopressin V2 receptor
• UniProt Gene Name: AVPR2
• UniProt Synonym Gene Names: ADHR; DIR; DIR3; V2R; V2R
• UniProt Entry Name: V2R_HUMAN

NCBI Description

This gene encodes the vasopressin receptor, type 2, also known as the V2 receptor, which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily, and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor, the V1a and V1b vasopressin receptors, the oxytocin receptor, and isotocin and mesotocin receptors in non-mammals, is well conserved, though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts, where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost, the disease Nephrogenic Diabetes Insipidus (NDI) results. The V2 receptor is also expressed outside the kidney although its tissue localization is uncertain. When these 'extrarenal receptors' are stimulated by infusion of a V2 selective agonist (dDAVP), a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known - its absence does not appear to be detrimental in NDI patients. The gene expression has also been described in fetal lung tissue and lung cancer associated with alternative splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

AVPR2: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption. Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD). This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis. Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI); also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter; Receptor, GPCR; Transporter, aquaporin family; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: Golgi apparatus; endoplasmic reticulum; integral to plasma membrane; integral to membrane; plasma membrane; endosome

Molecular Function: protein binding; peptide binding; vasopressin receptor activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; water transport; adenylate cyclase activation; positive regulation of systemic arterial blood pressure; excretion; cellular response to hormone stimulus; G-protein signaling, coupled to cAMP nucleotide second messenger; telencephalon development; regulation of systemic arterial blood pressure by vasopressin; positive regulation of protein ubiquitination; hemostasis; response to cytokine stimulus; positive regulation of cell proliferation; interferon-gamma production; positive regulation of vasoconstriction; renal water homeostasis; transmembrane transport

Disease: Nephrogenic Syndrome Of Inappropriate Antidiuresis; Diabetes Insipidus, Nephrogenic, X-linked