Typical Testing Data/Standard Curve (for reference only)
Human NAP ELISA Kit | NAP elisa kit
Human NAP (Neutrophil Alkaline Phosphatase) ELISA Kit
Gene Names
NAP1L1; NRP; NAP1; NAP1L
Reactivity
Human
Synonyms
NAP; Human NAP (Neutrophil Alkaline Phosphatase) ELISA Kit; NAP elisa kit
Reactivity
Human
Specificity
This kit recognizes natural and recombinantHumanNAP. No significant cross-reactivity or interference between HumanNAP and analogues was observed.
Sequence Length
391
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NAP elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanNAPconcentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NAPand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NAP.You can calculate the concentration of NAPin the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NAPand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NAP.You can calculate the concentration of NAPin the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,345 Da
NCBI Official Full Name
NAP
NCBI Official Synonym Full Names
nucleosome assembly protein 1-like 1
NCBI Official Symbol
NAP1L1
NCBI Official Synonym Symbols
NRP; NAP1; NAP1L
NCBI Protein Information
nucleosome assembly protein 1-like 1; HSP22-like protein interacting protein; NAP-1 related protein; NAP-1-related protein; hNRP
UniProt Protein Name
Nucleosome assembly protein 1-like 1
Protein Family
UniProt Gene Name
NAP1L1
UniProt Synonym Gene Names
NRP; hNRP
UniProt Entry Name
NP1L1_HUMAN
NCBI Description
This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing of this gene results in several transcript variants; however, not all have been fully described. [provided by RefSeq, Jul 2008]
Uniprot Description
NAP1L1: May be involved in modulating chromatin formation and contribute to regulation of cell proliferation. Belongs to the nucleosome assembly protein (NAP) family.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 12q21.2
Cellular Component: membrane; melanosome; nucleus
Molecular Function: protein binding
Biological Process: nucleosome assembly; positive regulation of cell proliferation; DNA replication
Research Articles on NAP
Similar Products
Product Notes
The Human NAP nap1l1 (Catalog #AAA2513894) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2513894 ELISA Kit recognizes Human NAP. It is sometimes possible for the material contained within the vial of "NAP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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