Typical Testing Data/Standard Curve (for reference only)
Guinea Pig ERalpha ELISA Kit | ERalpha elisa kit
Guinea pig ERalpha (Estrogen Receptor Alpha) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ERalpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ERalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ERalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ERalpha. You can calculate the concentration of ERalpha in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an estrogen receptor, a member of the nuclear hormone family of intracellular receptors. The encoded protein, activated by the sex hormone estrogen, is a transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Similar genes in human have been implicated in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Uniprot Description
ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.
Protein type: DNA-binding; Nuclear receptor
Cellular Component: Golgi apparatus; neuron projection; protein complex; membrane; mitochondrion; perinuclear region of cytoplasm; T-tubule; cytoplasm; plasma membrane; terminal button; perikaryon; nucleus; cytosol
Molecular Function: identical protein binding; ligand-dependent nuclear receptor activity; estrogen receptor activity; hormone binding; zinc ion binding; type 1 metabotropic glutamate receptor binding; metal ion binding; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; ATPase binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; chromatin binding; lipid binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; estrogen receptor signaling pathway; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; uterus development; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; positive regulation of epidermal growth factor receptor signaling pathway; regulation of neuron apoptosis; epithelial cell development; cell growth; steroid hormone receptor signaling pathway; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; antral ovarian follicle growth; negative regulation of transcription factor activity; positive regulation of nitric-oxide synthase activity; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; Sertoli cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription from RNA polymerase II promoter; negative regulation of glucose import; response to estrogen stimulus; negative regulation of mitosis; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of transcription factor activity
Research Articles on ERalpha
Similar Products
Product Notes
The Guinea Pig ERalpha esr1 (Catalog #AAA2513243) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2513243 ELISA Kit recognizes Guinea Pig ERalpha. It is sometimes possible for the material contained within the vial of "ERalpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
