Typical Testing Data/Standard Curve (for reference only)
Mouse Estrogen receptor ELISA Kit | Esr1 elisa kit
Mouse Estrogen receptor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ER. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ER and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ER in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an estrogen receptor, a member of the nuclear hormone family of intracellular receptors. The encoded protein, activated by the sex hormone estrogen, is a transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Similar genes in human have been implicated in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Uniprot Description
ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.
Protein type: Nuclear receptor; DNA-binding
Cellular Component: cytoplasm; cytosol; Golgi apparatus; membrane; mitochondrion; neuron projection; nuclear chromatin; nucleus; perikaryon; perinuclear region of cytoplasm; plasma membrane; protein complex; T-tubule; terminal button
Molecular Function: ATPase binding; beta-catenin binding; chromatin binding; DNA binding; enzyme binding; estrogen receptor activity; estrogen response element binding; hormone binding; identical protein binding; ligand-dependent nuclear receptor activity; lipid binding; metal ion binding; protein binding; protein complex binding; sequence-specific DNA binding; steroid binding; steroid hormone receptor activity; transcription factor activity; transcription factor binding; type 1 metabotropic glutamate receptor binding; zinc ion binding
Biological Process: androgen metabolic process; antral ovarian follicle growth; cell growth; elevation of cytosolic calcium ion concentration; epithelial cell development; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); male gonad development; negative regulation of glucose import; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of mitosis; negative regulation of smooth muscle cell proliferation; negative regulation of transcription factor activity; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of inflammatory response; regulation of neuron apoptosis; regulation of toll-like receptor signaling pathway; regulation of transcription, DNA-dependent; response to estradiol stimulus; response to estrogen stimulus; Sertoli cell proliferation; steroid hormone mediated signaling; steroid hormone receptor signaling pathway; transcription from RNA polymerase II promoter; transcription, DNA-dependent; uterus development; vagina development
Research Articles on Esr1
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Product Notes
The Mouse Esr1 esr1 (Catalog #AAA2885232) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2885232 ELISA Kit recognizes Mouse Esr1. It is sometimes possible for the material contained within the vial of "Estrogen receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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