Rabbit SBEM ELISA Kit | SBEM elisa kit
Rabbit SBEM (Small Breast Epithelial Mucin) ELISA Kit
Gene Names
MUCL1; SBEM
Reactivity
Rabbit
Synonyms
SBEM; Rabbit SBEM (Small Breast Epithelial Mucin) ELISA Kit; SBEM elisa kit
Reactivity
Rabbit
Specificity
This kit recognizes natural and recombinant Rabbit SBEM. No significant cross-reactivity or interference between Rabbit SBEM and analogues was observed.
Sequence Length
90
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
125-8000pg/mL
Sensitivity
Min: 75pg/mL; Max: 8000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SBEM elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit SBEM concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SBEM. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SBEM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SBEM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SBEM. You can calculate the concentration of SBEM in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SBEM. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SBEM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SBEM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SBEM. You can calculate the concentration of SBEM in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
9,039 Da
NCBI Official Full Name
small breast epithelial mucin
NCBI Official Synonym Full Names
mucin-like 1
NCBI Official Symbol
MUCL1
NCBI Official Synonym Symbols
SBEM
NCBI Protein Information
mucin-like protein 1; small breast epithelial mucin
UniProt Protein Name
Mucin-like protein 1
UniProt Gene Name
MUCL1
UniProt Synonym Gene Names
SBEM
UniProt Entry Name
MUCL1_HUMAN
Uniprot Description
MUCL1: May play a role as marker for the diagnosis of metastatic breast cancer.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q
Cellular Component: membrane; Golgi lumen; extracellular region
Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; post-translational protein modification
