Human Permeability Glycoprotein (Pgp) ELISA Kit | Pgp elisa kit

Human Permeability Glycoprotein (Pgp) ELISA Kit

Cat. Num. AAA455732

• Gene Names: PGP; AUM; G3PP; PGPase
• Reactivity: Human
• Synonyms: Permeability Glycoprotein (Pgp); Human Permeability Glycoprotein (Pgp) ELISA Kit; CD243; ABCB1; ABC20; CLCS; GP170; MDR1; PGY1; P-Glycoprotein; Multidrug resistance protein 1; ATP-Binding Cassette; Sub-Family B(MDR/TAP); Member 1; Colchicin Sensitivity; Pgp elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGLYRP2. No significant cross-reactivity or interference between PGLYRP2 and analogues was observed.
• Sequence Length: 321

• Samples: Human Serum, Plasma, Tissue Homogenates, Urine Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.62ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PGLYRP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PGLYRP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Pgp elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PGLYRP2 in human serum, plasma, tissue homogenates, urine or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGLYRP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PGLYRP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PGLYRP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PGLYRP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 108796653
• NCBI GeneID: 283871
• NCBI Accession #: NP_001035830.1
• NCBI GenBank Nucleotide #: NM_001042371.2
• UniProt Accession #: A6NDG6
• Molecular Weight: 34,006 Da
• NCBI Official Full Name: glycerol-3-phosphate phosphatase
• NCBI Official Synonym Full Names: phosphoglycolate phosphatase
• NCBI Official Symbol: PGP
• NCBI Official Synonym Symbols: AUM; G3PP; PGPase
• NCBI Protein Information: glycerol-3-phosphate phosphatase
• UniProt Protein Name: Glycerol-3-phosphate phosphatase
• Protein Family: Glycerol-3-phosphate phosphatase
• UniProt Gene Name: PGP
• UniProt Synonym Gene Names: PGP

Uniprot Description

Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed:26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP ().