Human CFTR ELISA Kit | CFTR elisa kit

Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) ELISA Kit

Cat. Num. AAA2508492

• Gene Names: CFTR; CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• Reactivity: Human
• Synonyms: CFTR; Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) ELISA Kit; CFTR elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human CFTR. No significant cross-reactivity or interference between Human CFTR and analogues was observed.
• Sequence Length: 13

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFTR elisa kit

Intended Uses||This ELISA kit applies to the in vitro quantitative determination of Human CFTR concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CFTR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CFTR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CFTR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CFTR. You can calculate the concentration of CFTR in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 180286
• NCBI GeneID: 1080
• UniProt Accession #: P13569; Q20BG8; Q20BH2; Q2I0A1; Q2I102
• Molecular Weight: 69,230 Da
• NCBI Official Full Name: CFTR, partial
• NCBI Official Synonym Full Names: cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
• NCBI Official Symbol: CFTR
• NCBI Official Synonym Symbols: CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• NCBI Protein Information: cystic fibrosis transmembrane conductance regulator; cAMP-dependent chloride channel; channel conductance-controlling ATPase
• UniProt Protein Name: Cystic fibrosis transmembrane conductance regulator
• Protein Family: Cystic fibrosis transmembrane conductance regulator
• UniProt Gene Name: CFTR
• UniProt Synonym Gene Names: ABCC7; CFTR
• UniProt Entry Name: CFTR_HUMAN

NCBI Description

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice

Protein type: Hydrolase; Membrane protein, integral; Channel, chloride; Transporter; Transporter, ABC family; EC 3.6.3.49; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: recycling endosome; protein complex; microvillus; cytoplasmic vesicle membrane; cell surface; early endosome membrane; basolateral plasma membrane; cytoplasm; apical plasma membrane; early endosome; plasma membrane

Molecular Function: bicarbonate transmembrane transporter activity; chloride channel activity; protein binding; enzyme binding; chloride channel inhibitor activity; chloride transmembrane transporter activity; channel-conductance-controlling ATPase activity; ATP-binding and phosphorylation-dependent chloride channel activity; ATP binding; PDZ domain binding

Biological Process: intracellular pH elevation; response to drug; response to peptide hormone stimulus; cholesterol transport; iodide transport; transepithelial chloride transport; water transport; membrane hyperpolarization; respiratory gaseous exchange; positive regulation of vasodilation; vasodilation; cholesterol biosynthetic process; cellular response to hormone stimulus; transport; response to estrogen stimulus; bicarbonate transport; response to cytokine stimulus; transmembrane transport; sperm capacitation; lung development

Disease: Vas Deferens, Congenital Bilateral Aplasia Of; Bronchiectasis With Or Without Elevated Sweat Chloride 1; Cystic Fibrosis; Pancreatitis, Hereditary