Typical Testing Data/Standard Curve (for reference only)
Porcine ET-1 ELISA Kit | ET-1 elisa kit
Porcine ET-1 (Endothelin 1) ELISA Kit
Gene Names
EDN1; PPET1
Reactivity
Porcine
Synonyms
ET-1; Porcine ET-1 (Endothelin 1) ELISA Kit; ET-1 elisa kit
Reactivity
Porcine
Specificity
This kit recognizes natural and recombinantPorcineET-1. No significant cross-reactivity or interference between PorcineET-1 and analogues was observed.
Sequence Length
202
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
12.5-800pg/mL
Sensitivity
Min: 7.5pg/mL; Max: 800pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ET-1 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of PorcineET-1concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ET-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ET-1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ET-1.You can calculate the concentration of ET-1in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ET-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ET-1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ET-1.You can calculate the concentration of ET-1in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,977 Da
NCBI Official Full Name
endothelin-1
NCBI Official Symbol
EDN1
NCBI Official Synonym Symbols
PPET1
NCBI Protein Information
endothelin-1; ET-1; gene for endothelin; preproendothelin-1
UniProt Protein Name
Endothelin-1
UniProt Gene Name
EDN1
UniProt Synonym Gene Names
ET-1; PPET1
UniProt Entry Name
EDN1_CANFA
Uniprot Description
Endothelins are endothelium-derived vasoconstrictor peptides.
Research Articles on ET-1
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Product Notes
The Porcine ET-1 edn1 (Catalog #AAA2508397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2508397 ELISA Kit recognizes Porcine ET-1. It is sometimes possible for the material contained within the vial of "ET-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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