Typical Testing Data/Standard Curve (for reference only)
Dog endothelin 1 ELISA Kit | EDN1 elisa kit
Dog Endothelin 1, ET-1 ELISA Kit
Gene Names
EDN1; PPET1
Reactivity
Dog
Synonyms
endothelin 1; Dog Endothelin 1; ET-1 ELISA Kit; ET1; HDLCQ7; preproendothelin 1; EDN1 elisa kit
Reactivity
Dog
Specificity
This assay has high sensitivity and excellent specificity for detection of Dog EDN1. No significant cross-reactivity or interference between Dog EDN1 and analogues was observed.
Sequence Length
202
Samples
Serum, plasma and tissue homogenates
Detection Range
3.12 pg/ml-200 pg/ml
Sensitivity
0.78 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EDN1 elisa kit
Introduction: Endothelin-1(ET-1), a peptide of 21 amino acid residues, is the most potent vasoconstrictive substance known. Originally isolated from aortic endothelial cells, ET-1 is now known to be one of a family of three mammalian vasoactive peptides that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3). These related peptides differ from ET-1 at two and six amino acid residue positions, respectively. A fourth peptide, vasoactive intestinal contractor (VIC), is sometimes classified as rat ET-2. All members of the endothelin family contain two essential disulfide bridges and six conserved amino acid residues at the C-terminus. Additionally, all of the endothelin family members are synthesized initially as prepropolypeptides of approximately 200 amino acid residues encoded by separate genes. The biochemistry and biology of the endothelins have been the subject of several reviews.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ET-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ET-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ET-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ET-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ET-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ET-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,977 Da
NCBI Official Full Name
endothelin-1
NCBI Official Symbol
EDN1
NCBI Official Synonym Symbols
PPET1
NCBI Protein Information
endothelin-1; ET-1; preproendothelin-1; gene for endothelin
UniProt Protein Name
Endothelin-1
Protein Family
UniProt Gene Name
EDN1
UniProt Synonym Gene Names
ET-1; PPET1
UniProt Entry Name
EDN1_CANFA
Uniprot Description
Function: Endothelins are endothelium-derived vasoconstrictor peptides.
Research Articles on EDN1
Similar Products
Product Notes
The Dog EDN1 edn1 (Catalog #AAA704122) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704122 ELISA Kit recognizes Dog EDN1. It is sometimes possible for the material contained within the vial of "endothelin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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