Monkey AMY2 ELISA Kit | AMY2 elisa kit
Monkey AMY2 (Amylase Alpha 2, Pancreatic) ELISA Kit
Gene Names
AMY2; alpha-amylase-like 2; AMY2; ARABIDOPSIS THALIANA ALPHA-AMYLASE-LIKE 2; ATAMY2; T23E18.6; T23E18_6
Reactivity
Monkey
Synonyms
AMY2; Monkey AMY2 (Amylase Alpha 2; Pancreatic) ELISA Kit; AMY2 elisa kit
Reactivity
Monkey
Specificity
This kit recognizes natural and recombinant Monkey AMY2. No significant cross-reactivity or interference between Monkey AMY2 and analogues was observed.
Sequence Length
413
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
3.125-200ng/mL
Sensitivity
Min: 1.875ng/mL; Max: 200ng/mL
Preparation and Storage
Store at 4 degree C.
Related Product Information for AMY2 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey AMY2 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AMY2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AMY2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AMY2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AMY2. You can calculate the concentration of AMY2 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AMY2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AMY2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AMY2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AMY2. You can calculate the concentration of AMY2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,170 Da
NCBI Official Full Name
alpha-amylase-like 2
NCBI Official Symbol
AMY2
NCBI Official Synonym Symbols
alpha-amylase-like 2; AMY2; ARABIDOPSIS THALIANA ALPHA-AMYLASE-LIKE 2; ATAMY2; T23E18.6; T23E18_6
NCBI Protein Information
alpha-amylase-like 2
UniProt Protein Name
Probable alpha-amylase 2
Protein Family
UniProt Gene Name
AMY2
UniProt Synonym Gene Names
AtAMY2
UniProt Entry Name
AMY2_ARATH
NCBI Description
alpha-amylase, putative / 1,4-alpha-D-glucan glucanohydrolase, putative, strong similarity to alpha-amylase GI:7532799 from (Malus x domestica);contains Pfam profile PF00128: Alpha amylase, catalytic domain. Predicted to be secreted based on SignalP analysis.
Uniprot Description
Probable alpha-amylase that does not seem to be required for breakdown of transitory starch in leaves.
