Typical Testing Data/Standard Curve (for reference only)
Sheep HO1 ELISA Kit | HO1 elisa kit
Sheep HO1 (Heme Oxygenase 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HO1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HO1. You can calculate the concentration of HO1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
catalyzes the oxidative cleavage of heme to biliverdin [RGD, Feb 2006]
Uniprot Description
HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family.
Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3
Cellular Component: endoplasmic reticulum membrane; perinuclear region of cytoplasm; endoplasmic reticulum; nucleolus; plasma membrane; caveola; cytosol; nucleus
Molecular Function: signal transducer activity; protein binding; enzyme binding; protein homodimerization activity; metal ion binding; phospholipase D activity; heme binding; oxidoreductase activity; heme oxygenase (decyclizing) activity
Biological Process: cell death; response to nicotine; negative regulation of smooth muscle cell proliferation; negative regulation of mast cell degranulation; positive regulation of smooth muscle cell proliferation; heme metabolic process; erythrocyte homeostasis; negative regulation of cell proliferation; regulation of transcription factor activity; heme catabolic process; small GTPase mediated signal transduction; phospholipid metabolic process; regulation of blood pressure; negative regulation of mast cell cytokine production; negative regulation of neuron apoptosis; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; healing during inflammatory response; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; negative regulation of DNA binding; positive regulation of angiogenesis; iron ion homeostasis; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to estrogen stimulus; response to hypoxia; response to oxidative stress
Research Articles on HO1
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Product Notes
The Sheep HO1 hmox1 (Catalog #AAA2503698) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2503698 ELISA Kit recognizes Sheep HO1. It is sometimes possible for the material contained within the vial of "HO1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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