anti-Mouse, Cow MFAP4 Polyclonal Antibody | anti-MFAP4 antibody

Anti-MFAP4 Antibody (aa228-255) IHC-plus

Cat. Num. AAA249935

• Reactivity: Mouse, Cow
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A purified
• Synonyms: MFAP4; Polyclonal Antibody; Anti-MFAP4 Antibody (aa228-255) IHC-plus; Rabbit Polyclonal to Human MFAP4; Human MFAP4; anti-MFAP4 antibody

• Host: MFAP4 antibody was produced in Rabbit
• Reactivity: Mouse, Cow
• Clonality: Polyclonal
• Specificity: This MFAP4 antibody is generated from rabbits immunized with KLH conjugated synthetic peptide between 228-255 amino acids from C-terminal region of human MFAP4.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% Sodium Azide
• Sequence Positions: aa228-255

• Applicable Applications for anti-MFAP4 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:10 - 1:50); IHC-P (1:50 - 1:100); WB (1:1000)
• Target: MFAP4
• Immunogen Species: MFAP4 antibody was raised against Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short Term: 2-8°C; Long Term: -20°C

Immunohistochemistry (IHC)

(Anti-MFAP4 antibody IHC staining of human tonsil, collagenous stroma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot of lysate from human lung tissue lysate, using MFAP4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

Flow Cytometry (FC/FACS)

(MFAP4 Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MFAP4 antibody

MFAP4 Antibody Description: MFAP4 is a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region. Two transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 2506403
• NCBI GeneID: 4239
• UniProt Accession #: P55083; Q6P680; A8KAJ1; A8MVM2; B4E317
• NCBI Official Full Name: Microfibril-associated glycoprotein 4
• NCBI Official Synonym Full Names: microfibrillar associated protein 4
• NCBI Official Symbol: MFAP4
• NCBI Protein Information: microfibril-associated glycoprotein 4
• UniProt Protein Name: Microfibril-associated glycoprotein 4
• Protein Family: Microfibril-associated glycoprotein
• UniProt Gene Name: MFAP4
• UniProt Entry Name: MFAP4_HUMAN

NCBI Description

This gene encodes a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

MFAP4: Could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 is deleted in the Smith-Magenis syndrome (SMS).

Protein type: Secreted; Secreted, signal peptide; Calcium-binding; Cell adhesion

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: extracellular matrix; extracellular region; microfibril

Molecular Function: protein binding

Biological Process: cell adhesion; elastic fiber assembly; extracellular matrix organization and biogenesis; fibril organization and biogenesis; UV protection