Rabbit anti-Human, Mouse MFAP4 Polyclonal Antibody | anti-MFAP4 antibody

MFAP4, CT (MFAP4, Microfibril-associated glycoprotein 4) (Biotin)

Cat. Num. AAA6320494

• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MFAP4; Polyclonal Antibody; CT (MFAP4; Microfibril-associated glycoprotein 4) (Biotin); Microfibril-associated glycoprotein 4; anti-MFAP4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MFAP4 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MFAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 225-255 from the C-terminal region of human MFAP4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MFAP4 Antibody (C-term) in mouse lung tissue lysates (35ug/lane). MFAP4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(MFAP4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFAP4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MFAP4 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MFAP4 antibody

MFAP4 is a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region.

Product Categories/Family for anti-MFAP4 antibody

Antibodies; Carbohydrates; Glycoproteins

References

Edelman, E.A., et al. Clin. Genet. 71(6):540-550(2007). Schlosser, A., et al. Scand. J. Immunol. 64(2):104-116(2006). Liu, T., et al. J. Proteome Res. 4(6):2070-2080(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 2506403
• NCBI GeneID: 4239
• UniProt Accession #: P55083; Q6P680; A8KAJ1; A8MVM2; B4E317
• Molecular Weight: 28,648 Da
• NCBI Official Full Name: Microfibril-associated glycoprotein 4
• NCBI Official Synonym Full Names: microfibrillar-associated protein 4
• NCBI Official Symbol: MFAP4
• NCBI Protein Information: microfibril-associated glycoprotein 4
• UniProt Protein Name: Microfibril-associated glycoprotein 4
• Protein Family: Microfibril-associated glycoprotein
• UniProt Gene Name: MFAP4
• UniProt Entry Name: MFAP4_HUMAN

NCBI Description

This gene encodes a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

MFAP4: Could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 is deleted in the Smith-Magenis syndrome (SMS).

Protein type: Calcium-binding; Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: extracellular matrix; microfibril; extracellular region

Molecular Function: protein binding

Biological Process: elastic fiber assembly; extracellular matrix organization and biogenesis; UV protection; fibril organization and biogenesis; cell adhesion