Mouse anti-Human CD32A Monoclonal Antibody | anti-CD32A antibody

Mouse Monoclonal [clone 13D7] (IgG2b) to Human CD32A

Cat. Num. AAA249188

• Gene Names: FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation
• Purity: Protein A/G Purified
• Synonyms: CD32A; Monoclonal Antibody; Mouse Monoclonal [clone 13D7] (IgG2b) to Human CD32A; Anti-CD32A Antibody (clone 13D7) IHC-plus; FCGR2A; CD32; CD32 antigen; CDw32; FCG2; FcGR; FCGR2A1; FcRII-a; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-c; IgG Fc receptor II-a; Igg fc receptor ii-c; Fc-gamma rii-c; FCGR2; Igg fc receptors ii-c; IGFR2; Human CD32A; anti-CD32A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 13D7
• Specificity: Human CD32A / CD32
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 1.09 mg/ml (varies by lot)
• Sequence Length: 316

• Applicable Applications for anti-CD32A antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IF (1:100), IHC-P (10 ug/ml), IP, WB (1:2000); Usage: Concentration: 0.5-1.0 mg/ml (Lot dependent)
• Target Species: Human
• Immunogen Description: Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Immunogen: CD32A antibody was raised against full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CD32A antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CD32A antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Anti-FCGR2A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.)

Immunoprecipitation (IP)

(Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.)

Related Product Information for anti-CD32A antibody

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 50511936
• NCBI GeneID: 2212
• NCBI Accession #: NP_067674.2
• NCBI GenBank Nucleotide #: NM_021642.3
• UniProt Accession #: P12318; Q8WUN1; Q8WW64
• Molecular Weight: 34,930 Da
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor II-a isoform 2
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIa, receptor (CD32)
• NCBI Official Symbol: FCGR2A
• NCBI Official Synonym Symbols: CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor II-a; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; Immunoglobulin G Fc receptor II
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor II-a
• UniProt Gene Name: FCGR2A
• UniProt Synonym Gene Names: CD32; FCG2; FCGR2A1; IGFR2; IgG Fc receptor II-a; Fc-gamma-RIIa; FcRII-a
• UniProt Entry Name: FCG2A_HUMAN

NCBI Description

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]

Uniprot Description

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: plasma membrane; integral to membrane

Molecular Function: IgG binding

Biological Process: innate immune response

Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To