Rabbit anti-Human FCGR2A Polyclonal Antibody | anti-FCGR2A antibody

FCGR2A, CT (FCGR2A, CD32, FCG2, FCGR2A1, IGFR2, Low affinity immunoglobulin gamma Fc region receptor II-a, CDw32, Fc-gamma RII-a, CD32) (PE)

Cat. Num. AAA6298636

• Gene Names: FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FCGR2A; Polyclonal Antibody; CT (FCGR2A; CD32; FCG2; FCGR2A1; IGFR2; Low affinity immunoglobulin gamma Fc region receptor II-a; CDw32; Fc-gamma RII-a; CD32) (PE); anti-FCGR2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-FCGR2A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FCGR2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-285 amino acids from the C-terminal region of human FCGR2A.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H292 cell line lysates (35ug/lane) usingMBS6002249.This demonstrates the FCGR2A antibody detected the FCGR2A protein (arrow).)

Western Blot (WB)

(Western Blot analysis of FCGR2A (arrow) usingMBS6002249. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FCGR2A gene.)

Related Product Information for anti-FCGR2A antibody

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-FCGR2A antibody

Antibodies; CD Markers

References

Dornan, D., et al. Blood 116(20):4212-4222(2010). Zhang, C.Y., et al. J. Biol. Chem. 285(44):34250-34258(2010). Iwasaki, M., et al. Breast Cancer Res. Treat. (2010). In press: Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010). In press: Sfar, I., et al. Arch Inst Pasteur Tunis 86 (1-4), 51-62 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 210031822
• NCBI GeneID: 2212
• NCBI Accession #: NP_001129691.1
• NCBI GenBank Nucleotide #: NM_001136219.1
• UniProt Accession #: P12318; Q8WUN1; Q8WW64
• Molecular Weight: 35,001 Da
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor II-a isoform 1
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIa, receptor (CD32)
• NCBI Official Symbol: FCGR2A
• NCBI Official Synonym Symbols: CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor II-a; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; Immunoglobulin G Fc receptor II
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor II-a
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: FCGR2A
• UniProt Synonym Gene Names: CD32; FCG2; FCGR2A1; IGFR2; IgG Fc receptor II-a; Fc-gamma-RIIa; FcRII-a
• UniProt Entry Name: FCG2A_HUMAN

NCBI Description

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]

Uniprot Description

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: plasma membrane; integral to membrane

Molecular Function: IgG binding

Biological Process: innate immune response

Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To